Dynamic Metabolite Profile Changes in Semen Ziziphi Spinosae During Ripening

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-03-19 DOI:10.1002/rcm.10024

Xi Tian, Sujun Zhang, Liqiang Gu, Wei Tian, Lingdi Liu, Qiang Li, Tao Jiang

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引用次数: 0

Abstract

Rationale

In traditional Chinese medicine, Semen Ziziphi Spinosae (SZS) is employed for alleviating conditions such as neurasthenia, sleep disorders, and anxiety. Its therapeutic effects are attributed to an abundance of biologically active compounds. The main objective of this study was the comparative profiling of SZS from different harvest times using a widely targeted metabolomics approach.

Methods

First, UPLC-Q Trap-MS/MS was used for identification of metabolic profile. Then, multivariate statistical analysis and KEGG enrichment analysis were performed to screen out the differential metabolites and related metabolic pathways among different growth stages.

Results

In total, 466 metabolites were identified at three different growth and development stages (T1, T2, and T3) of SZS using UPLC-Q Trap-MS/MS, including 83 flavonoids, 80 phenolic acids, 67 amino acids and derivatives, 56 lipids, 39 nucleotides and derivatives, 38 organic acids, 1 quinone, 6 lignans and coumarins, 53 other metabolites, 10 tannins, 20 alkaloids, and 13 terpenoids. The result of clustering and PCA analyses showed that there was a great difference in metabolites between SZS at three growth stages. Differential metabolites in three comparison groups (T1 vs. T2, T2 vs. T3, and T1 vs. T3) were 195, 104, and 96, respectively. There were 29 common differential metabolites among the three different growth stages of SZS. The contents of important active ingredients (flavonoids and terpenoids) gradually increased during the T1, T2, and T3 stages, indicating that SZS harvested during T3 period was suitable for medicinal use. All the differential metabolites screened were enriched in 11 metabolic pathways, including glycerolipid metabolism, glycerophospholipid metabolism, phenylalanine metabolism, and phenylpropanoid biosynthesis pathway.

Conclusions

This study provides a more comprehensive understanding of the dynamic changes in the metabolic profile of SZS, laying a foundation for subsequent development and utilization.

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酸枣精液成熟过程中动态代谢谱的变化

在传统中医中，紫皮精（SZS）被用来缓解神经衰弱、睡眠障碍和焦虑等疾病。其治疗效果归因于丰富的生物活性化合物。本研究的主要目的是利用广泛靶向的代谢组学方法对不同收获时间的SZS进行比较分析。方法首先采用UPLC-Q Trap-MS/MS进行代谢谱鉴定。然后进行多元统计分析和KEGG富集分析，筛选不同生长阶段的差异代谢物及相关代谢途径。结果利用ulc - q陷阱-MS/MS技术，共鉴定出SZS在T1、T2和T3三个不同生长发育阶段的466种代谢物，包括83种黄酮类、80种酚酸、67种氨基酸及其衍生物、56种脂类、39种核苷酸及其衍生物、38种有机酸、1种醌、6种木脂素和香豆素、53种其他代谢物、10种单宁、20种生物碱和13种萜类。聚类分析和主成分分析结果表明，3个生育期稻穗间代谢物含量存在较大差异。三个对照组（T1与T2、T2与T3、T1与T3）的差异代谢物分别为195、104和96。3个不同生长阶段的稻瘟病菌共有29种差异代谢物。主要有效成分黄酮类化合物和萜类化合物的含量在T1、T2和T3阶段逐渐增加，表明T3阶段收获的稻穗适合药用。所有筛选的差异代谢物均富集于11条代谢途径，包括甘油脂代谢、甘油磷脂代谢、苯丙氨酸代谢和苯丙素生物合成途径。结论本研究更全面地了解了SZS代谢谱的动态变化，为后续的开发利用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.