Simple and sensitive SERS platform for Staphylococcus aureus one-pot determination by photoactivated CRISPR/Cas12a cascade system and core–shell DNA tetrahedron@AuNP@Fe3O4 reporter

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-03-18 DOI:10.1007/s00604-025-07098-w

Rui Fan, Shihua Luo, Yangfen He, Yunju Xiao, Yuxin Liang, Lifeng Zhang, Wenbin Li, Ye Zhang, Ling Li

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引用次数: 0

Abstract

Staphylococcus aureus (S. aureus) is a widely prevalent Gram-positive bacteria that can cause serious infections and diseases in humans and other organisms. Timely detection and treatment in clinical settings is crucial for patient safety and public health. However, current methods for S. aureus detection still face some limitations, such as time-consuming operation, false positives, and labor-intensive available methodology with low sensitivity. Therefore, it is particularly important to develop a rapid, simple, sensitive, and cost-effective method for detecting S. aureus. We developed a SERS platform based on allosteric aptamer-triggered catalytic hairpin assembly (CHA) and photoactivated CRISPR/Cas12a reactions, combined with a multifunctional core–shell structure as the SERS reporter, enabling highly sensitive one-pot determination of S. aureus. Compared with traditional two-step and one-pot analysis methods, this strategy offers superior sensitivity and can successfully identify real samples contaminated with S. aureus. The platform utilizes light-controlled CHA and CRISPR/Cas12a reactions, effectively preventing interference between different reaction systems. Therefore, the photoactivated one-pot CHA/Cas12a strategy provides a simple, rapid, highly sensitive, specific, and cost-effective method for one-pot determination of S. aureus in clinical samples.

Graphical abstract

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光激活CRISPR/Cas12a级联系统和核壳DNA一锅检测金黄色葡萄球菌简单灵敏的SERS平台tetrahedron@AuNP@Fe3O4报告基因

金黄色葡萄球菌（金黄色葡萄球菌）是一种广泛流行的革兰氏阳性细菌，可引起人类和其他生物的严重感染和疾病。在临床环境中及时发现和治疗对患者安全和公共卫生至关重要。然而，目前的金黄色葡萄球菌检测方法仍存在一些局限性，如操作时间长、假阳性、现有方法劳动强度大、灵敏度低等。因此，开发一种快速、简便、灵敏、经济的金黄色葡萄球菌检测方法显得尤为重要。我们开发了一个基于变构适配体触发的催化发夹组装（CHA）和光激活CRISPR/Cas12a反应的SERS平台，结合多功能核壳结构作为SERS报告基因，实现了金黄色葡萄球菌的高灵敏度一锅检测。与传统的两步和一锅分析方法相比，该方法具有更高的灵敏度，可以成功地鉴定出金黄色葡萄球菌污染的真实样品。该平台利用光控CHA和CRISPR/Cas12a反应，有效防止不同反应体系之间的干扰。因此，光激活一锅CHA/Cas12a策略为临床样品中金黄色葡萄球菌的一锅检测提供了一种简单、快速、高灵敏度、特异性和高性价比的方法。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.