Dynamic abundances of m7G, m5C and m1A mRNA modifications are associated with tomato fruit quality following harvest

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-03-18 DOI:10.1016/j.postharvbio.2025.113522

Ziyi Zhao , Shuang Zeng , Huijie Liu , Mingjia Chen , Dongdong Li , Kunsong Chen

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引用次数: 0

Abstract

Ribonucleic acid (RNA) modifications play diverse roles in living organisms, and accumulating evidence reveals their significance in fruit development and ripening. However, the extent of their involvement in regulating postharvest fruit quality remains largely unknown. In this study, we characterized typical changes of tomato fruit following one-week storage, in which fruit got more pigmentation, lose fresh weight, reduced firmness and ethylene production, accompanied by decreased expression of genes related to these processes. We profiled the abundances of seven types of RNA modifications in tomato fruit at the beginning and end of the storage period, by digesting RNAs and quantifying individual nucleoside in LC-MS/MS. We observed dynamic changes of N⁷-methylguanosine (m⁷G), 5-methylcytosine (m⁵C) and N¹-methyladenosine (m¹A) mRNA modifications during postharvest storage. Furthermore, we noted a significant decrease in the expression of genes encoding enzymes associated with m⁷G (SlMETTL1, SlMETTL2 and SleIF4E3), m⁵C (SlTRDMT and SlALYREF2, SlYTHDC1), and m¹A (SlYTHDC2A and SlALKBH2) mRNA modifications over the course of fruit storage. Our results unveil the dynamic alterations in mRNA modifications occurring during postharvest tomato fruit storage and imply that these modifications may play roles in regulating the expression of genes contributing to fruit quality.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.