Chitosan-based fluorescent nanocarriers: A novel drug delivery strategy for oral squamous cell carcinoma therapy

IF 2.4 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Gang Zhu , Qiang Ruan , Zhonghui Tian , Fengxia Liu , Luyan Guo , Zhongrui Zhang
{"title":"Chitosan-based fluorescent nanocarriers: A novel drug delivery strategy for oral squamous cell carcinoma therapy","authors":"Gang Zhu ,&nbsp;Qiang Ruan ,&nbsp;Zhonghui Tian ,&nbsp;Fengxia Liu ,&nbsp;Luyan Guo ,&nbsp;Zhongrui Zhang","doi":"10.1016/j.carres.2025.109459","DOIUrl":null,"url":null,"abstract":"<div><div>Oral squamous cell carcinoma (OSCC) accounts for over 90 % of all oral cancers, underscoring the urgent need for effective treatment strategies to improve patient survival. Grape seed polyphenols (GSP), a naturally occurring plant-derived compound, have shown promise as a therapeutic agent for OSCC. However, their clinical application is limited by poor solubility and instability. To address these challenges, coordination polymers (CPs) were employed as drug carriers, enhancing GSP's solubility, bioavailability, and controlled release. In this study, compound 1 (CP1) was synthesized and incorporated with GSP (CP1@1@GSP), significantly improving drug encapsulation efficiency (over 50 %) and drug loading (16 %), ensuring more effective drug delivery. Despite these advantages, concerns about metal ion release and potential immune responses necessitate further safety evaluation. To mitigate these risks, chitosan (CS), a biocompatible and low-toxicity natural polymer, was introduced. The development of pyrene-modified chitosan-based hollow nanoparticles (Pyrene-CS@CP1@1@GSP) facilitated both drug delivery and fluorescence-based real-time tracking. Characterization using scanning electron microscopy (SEM) and dynamic light scattering (DLS) confirmed the uniform spherical morphology of the nanoparticles, with an average size of approximately 150 nm, stable dispersion, a low polydispersity index (PDI), and excellent self-assembly properties. Further functional evaluation revealed that Pyrene-CS@CP1@1@GSP effectively modulates glycolysis in OSCC cells. Treatment significantly inhibited OSCC cell proliferation in a dose-dependent manner, with glucose levels in the cell supernatant increasing significantly (p &lt; 0.05), indicating reduced glucose uptake by cancer cells. Simultaneously, lactic acid levels decreased (p &lt; 0.05), suggesting suppression of glycolytic activity. Additionally, fluorescence quenching and subsequent restoration of pyrene fluorescence during drug release enabled real-time tracking of drug distribution. These findings demonstrate that Pyrene-CS@CP1@1@GSP enhances the bioavailability and stability of GSP while effectively regulating glycolysis, thereby influencing OSCC progression. This study presents a promising strategy for targeted OSCC therapy, offering both improved treatment efficacy and real-time drug release monitoring, with potential applications in future clinical settings.</div></div>","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"552 ","pages":"Article 109459"},"PeriodicalIF":2.4000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Research","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0008621525000850","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Oral squamous cell carcinoma (OSCC) accounts for over 90 % of all oral cancers, underscoring the urgent need for effective treatment strategies to improve patient survival. Grape seed polyphenols (GSP), a naturally occurring plant-derived compound, have shown promise as a therapeutic agent for OSCC. However, their clinical application is limited by poor solubility and instability. To address these challenges, coordination polymers (CPs) were employed as drug carriers, enhancing GSP's solubility, bioavailability, and controlled release. In this study, compound 1 (CP1) was synthesized and incorporated with GSP (CP1@1@GSP), significantly improving drug encapsulation efficiency (over 50 %) and drug loading (16 %), ensuring more effective drug delivery. Despite these advantages, concerns about metal ion release and potential immune responses necessitate further safety evaluation. To mitigate these risks, chitosan (CS), a biocompatible and low-toxicity natural polymer, was introduced. The development of pyrene-modified chitosan-based hollow nanoparticles (Pyrene-CS@CP1@1@GSP) facilitated both drug delivery and fluorescence-based real-time tracking. Characterization using scanning electron microscopy (SEM) and dynamic light scattering (DLS) confirmed the uniform spherical morphology of the nanoparticles, with an average size of approximately 150 nm, stable dispersion, a low polydispersity index (PDI), and excellent self-assembly properties. Further functional evaluation revealed that Pyrene-CS@CP1@1@GSP effectively modulates glycolysis in OSCC cells. Treatment significantly inhibited OSCC cell proliferation in a dose-dependent manner, with glucose levels in the cell supernatant increasing significantly (p < 0.05), indicating reduced glucose uptake by cancer cells. Simultaneously, lactic acid levels decreased (p < 0.05), suggesting suppression of glycolytic activity. Additionally, fluorescence quenching and subsequent restoration of pyrene fluorescence during drug release enabled real-time tracking of drug distribution. These findings demonstrate that Pyrene-CS@CP1@1@GSP enhances the bioavailability and stability of GSP while effectively regulating glycolysis, thereby influencing OSCC progression. This study presents a promising strategy for targeted OSCC therapy, offering both improved treatment efficacy and real-time drug release monitoring, with potential applications in future clinical settings.

Abstract Image

基于壳聚糖的荧光纳米载体:一种治疗口腔鳞状细胞癌的新型药物递送策略
口腔鳞状细胞癌(OSCC)占所有口腔癌的90%以上,迫切需要有效的治疗策略来提高患者的生存率。葡萄籽多酚(GSP)是一种天然存在的植物衍生化合物,已显示出作为OSCC治疗剂的前景。然而,其溶解性差和不稳定性限制了其临床应用。为了解决这些问题,采用配位聚合物(CPs)作为药物载体,提高GSP的溶解度、生物利用度和控释。本研究合成了化合物1 (CP1)并与GSP (CP1@1@GSP)结合,显著提高了药物包封效率(50%以上)和载药量(16%),保证了更有效的给药。尽管有这些优点,但对金属离子释放和潜在免疫反应的担忧需要进一步的安全性评估。为了减轻这些风险,壳聚糖(CS)是一种生物相容性和低毒的天然聚合物。吡啶改性壳聚糖基中空纳米颗粒(Pyrene-CS@CP1@1@GSP)的开发促进了药物传递和基于荧光的实时跟踪。通过扫描电子显微镜(SEM)和动态光散射(DLS)表征,证实了纳米颗粒的均匀球形形貌,平均尺寸约为150 nm,分散稳定,多分散指数(PDI)低,自组装性能优异。进一步的功能评估显示Pyrene-CS@CP1@1@GSP有效调节OSCC细胞中的糖酵解。治疗显著抑制OSCC细胞增殖呈剂量依赖性,细胞上清中的葡萄糖水平显著升高(p <;0.05),表明癌细胞对葡萄糖的摄取减少。同时,乳酸水平降低(p <;0.05),提示糖酵解活性受到抑制。此外,在药物释放过程中,荧光猝灭和随后的芘荧光恢复使得实时跟踪药物分布成为可能。这些发现表明Pyrene-CS@CP1@1@GSP增强GSP的生物利用度和稳定性,同时有效调节糖酵解,从而影响OSCC的进展。本研究提出了一种有前景的OSCC靶向治疗策略,既能提高治疗效果,又能实时监测药物释放,在未来的临床环境中具有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Carbohydrate Research
Carbohydrate Research 化学-生化与分子生物学
CiteScore
5.00
自引率
3.20%
发文量
183
审稿时长
3.6 weeks
期刊介绍: Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信