Ectopic expression of Dryopteris fragrans DfMTPSL6, a directly target gene of DfWRKY16/45, enhanced drought tolerance in tobacco plants

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-14 DOI:10.1016/j.plaphy.2025.109786

Dongrui Zhang, Xun Tang, Xiaojie Qiu, Jiameng Su, Qian Ma, Yongjia Li, Ying Chang

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引用次数: 0

Abstract

Terpenoid synthesis in seed plants is primarily catalyzed by Typical Terpene Synthase (TPS) enzymes. However, terrestrial non-seed plants also possess microbial terpene synthase-like (MTPSL) enzymes for terpene synthesis. A previous study has demonstrated the presence of both TPSs and MTPSLs in Dryopteris fragrans (L.) Schott. Specifically, DfMTPSL6 has been identified as the enzyme responsible for catalyzing the conversion of farnesyl diphosphate (FPP) to nerolidol. Nerolidol has several functions, including insect, disease and chilling resistance, although its biological role in eukaryotes remains to be confirmed. Transcription factors regulate the terpenoid biosynthesis by binding to gene promoters. However, the regulation of terpene metabolism by transcription factors, including MTPSLs, has not been investigated in ferns.

This study analyzed the conservation of DfMTPSL6, a nerolidol synthase, expressed in tobacco plants to enhance drought tolerance. Promoter analysis revealed specific expression in glandular hairs, with the active site responsive to MeJA and PEG treatments and containing a W-box, a binding site for WRKY transcription factors. 48 DfWRKY transcription factors were identified, and their expression patterns under MeJA and PEG treatments were analyzed. Yeast one-hybrid assays identified DfWRKY16 and DfWRKY45 as potential regulators of DfMTPSL6. Subcellular localization and transcriptional activation analysis confirmed that DfWRKY16 and DfWRKY45 are transcriptional activator and promotion of DfMTPSL6 expression.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.