Changes in absorption spectra of indocyanine green after visible light exposure and cold dark storage

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-12 DOI:10.1016/j.saa.2025.126048

Magdalena Szpunar , David Aebisher , Andrzej Wal

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Abstract

Indocyanine green (ICG) is commonly used as a fluorescent cardiovascular imaging agent, but its stability under different conditions remains insufficiently studied. This research examines the photochemical stability and aggregation behaviour of ICG in aqueous solutions subjected to visible light irradiation and during cold, dark storage. ICG solutions at different concentrations were exposed to visible light, and changes in absorption spectra were monitored over time. Exponential decreases in the absorption maxima were observed for both monomeric and dimeric ICG during irradiation. These changes were modelled, allowing for the description of decay processes caused by irradiation for monomers and dimmers, respectively. Furthermore, after a month of dark storage at 4 °C, a decrease in absorption was observed, accompanied by the formation of J-aggregates. These results indicate that ICG undergoes significant structural changes that may affect its fluorescence properties, with potential applications for use in photodynamic therapy. More research is needed to explore the impact of these changes on the performance of ICG in vivo.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.