Characterization of phage vB_EcoP_HC25 and its therapeutic effect on chicken colibacillosis

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-03-17 DOI:10.1016/j.micpath.2025.107490

Heping Li , Danying Xie , Ruochao Huang , Bowen Shi , Jinghao Xie , Chen Yang , Xinyan Yao , Chaoxiang Jia , Guoyu Yang , Shuang Guo , Yueying Wang

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Abstract

Bacteriophage therapy has proved to be an attractive candidate for decreasing drug-resistant bacteria. In this study, a novel E. coli phage vB_EcoP_HC25 was isolated from sewage. Phage vB_EcoP_HC25 has an elongated head and a short tail, exhibiting the typical features of the rare C3 morphotype. The burst size was approximately 10⁵ PFU/cell. Phage vB_EcoP_HC25 exhibited good pH range (pH 5–11) and temperature tolerance (<60 °C). The genomic analysis revealed that phage vB_EcoP_HC25 does not carry any virulence factor genes or antibiotic resistance genes. Moreover, it was found that vB_EcoP_HC25 is a promising candidate phage for biocontrol against antibiotic-resistant E. coli in milk and chicken meat. In addition, the results of the mental state, behavior, serum inflammatory factors, and intestinal morphology analyses indicated that phage vB_EcoP_HC25 was effective to control colibacillosis in chicks infected with E. coli.

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)