Identification of lncRNA biomarkers for keloid diagnosis and functional characterization of CPEB2-AS1

IF 3.2 3区医学 Q2 CRITICAL CARE MEDICINE

Burns Pub Date : 2025-03-05 DOI:10.1016/j.burns.2025.107448

Shengjing Xu , Yali Jiang , Xianggong Zhu , Yanting Rao , Min Zhang , Yan Lu

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引用次数: 0

Abstract

Background

Keloids are pathological scars marked by excessive tissue growth, and their diagnosis currently depends on clinical observation, lacking objective biomarkers. Although the regulatory role of long non-coding RNAs (lncRNAs) in various diseases has drawn attention, their function in keloids remains unclear.

Methods

This study employed high-throughput sequencing, bioinformatics analysis and machine learning algorithms to identify differentially expressed lncRNAs in keloid tissues and to construct a diagnostic model. The relationship between these lncRNAs and immune responses, as well as gene expression regulation, was explored through immune signature analysis and co-expression networks. The role of CPEB2-AS1 in keloids was investigated through expression validation, pan-cancer analysis and functional experiments.

Results

Four key differentially expressed lncRNAs were identified, enabling the development of an effective diagnostic model that distinguishes keloid tissues from healthy controls. Immune signature analysis revealed a positive correlation between CPEB2-AS1 and immune cell activity. Co-expression networks and functional enrichment analysis suggested that these lncRNAs may regulate RNA processing and nucleic acid binding. Expression validation and pan-cancer analysis confirmed their abnormal expression in keloids. Functional experiments demonstrated that CPEB2-AS1 significantly influences cell proliferation, migration, invasion and apoptosis.

Conclusion

This study identifies new molecular markers for the early diagnosis of keloids and highlights the potential role of CPEB2-AS1 in keloid progression, offering insights for future therapeutic strategies.

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来源期刊

Burns 医学-皮肤病学

CiteScore

4.50

自引率

18.50%

发文量

304

审稿时长

72 days

期刊介绍： Burns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns. The journal focuses on clinical, scientific and social aspects of these injuries and covers the prevention of the injury, the epidemiology of such injuries and all aspects of treatment including development of new techniques and technologies and verification of existing ones. Regular features include clinical and scientific papers, state of the art reviews and descriptions of burn-care in practice. Topics covered by Burns include: the effects of smoke on man and animals, their tissues and cells; the responses to and treatment of patients and animals with chemical injuries to the skin; the biological and clinical effects of cold injuries; surgical techniques which are, or may be relevant to the treatment of burned patients during the acute or reconstructive phase following injury; well controlled laboratory studies of the effectiveness of anti-microbial agents on infection and new materials on scarring and healing; inflammatory responses to injury, effectiveness of related agents and other compounds used to modify the physiological and cellular responses to the injury; experimental studies of burns and the outcome of burn wound healing; regenerative medicine concerning the skin.