A Golgi apparatus-targeted ratiometric fluorescent probe for HOCl and its applications for anti-inflammatory evaluation of Dachengqi Decoction

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-11 DOI:10.1016/j.saa.2025.126026

Li Yang , Xuan Zhao , Yan Liu , Ye-Xin Liao , Yuyu Fang , Ji-Ting Hou , Shan Wang

{"title":"A Golgi apparatus-targeted ratiometric fluorescent probe for HOCl and its applications for anti-inflammatory evaluation of Dachengqi Decoction","authors":"Li Yang , Xuan Zhao , Yan Liu , Ye-Xin Liao , Yuyu Fang , Ji-Ting Hou , Shan Wang","doi":"10.1016/j.saa.2025.126026","DOIUrl":null,"url":null,"abstract":"<div><div>Verifying the fluctuations of reactive oxidative species in the Golgi apparatus (GA) is crucial to investigate the pathology in inflammation. In this work, we present a fluorescent probe <strong>GA-PBC</strong> based on the phenothiazine-coumarin chromophore with a benzenesulfonamide unit as the GA target. <strong>GA-PBC</strong> manifests a red fluorescence peak at 620 nm, while it shifts to 510 nm upon reaction with HOCl, thus facilitating a ratiometric sensing manner. The probe shows high selectivity, superb sensitivity (limit of detection: 85.8 nM), rapid response (within seconds), and predominant accumulation in the GA. Intracellular imaging tests demonstrates the ability of <strong>GA-PBC</strong> to indicate the concentration changes of HOCl in live cells. Especially, it can be utilized to identify the active ingredients of Dachengqi Decoction (a kind of Traditional Chinese Medicine formula) in inflamed cells using HOCl in the GA as biomarker, suggesting that this probe is potentially useful for the evaluation of anti-oxidation efficacy of natural medicine.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"336 ","pages":"Article 126026"},"PeriodicalIF":4.3000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142525003324","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}

引用次数: 0

Abstract

Verifying the fluctuations of reactive oxidative species in the Golgi apparatus (GA) is crucial to investigate the pathology in inflammation. In this work, we present a fluorescent probe GA-PBC based on the phenothiazine-coumarin chromophore with a benzenesulfonamide unit as the GA target. GA-PBC manifests a red fluorescence peak at 620 nm, while it shifts to 510 nm upon reaction with HOCl, thus facilitating a ratiometric sensing manner. The probe shows high selectivity, superb sensitivity (limit of detection: 85.8 nM), rapid response (within seconds), and predominant accumulation in the GA. Intracellular imaging tests demonstrates the ability of GA-PBC to indicate the concentration changes of HOCl in live cells. Especially, it can be utilized to identify the active ingredients of Dachengqi Decoction (a kind of Traditional Chinese Medicine formula) in inflamed cells using HOCl in the GA as biomarker, suggesting that this probe is potentially useful for the evaluation of anti-oxidation efficacy of natural medicine.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.