Photobiomodulation therapy facilitates transplantation of dental pulp stem cells for spinal cord injury

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy Pub Date : 2025-03-14 DOI:10.1016/j.pdpdt.2025.104559

Xinran Zhang , Wenwen Yang , Biao Zhu , Lin Su , Haotian Li

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引用次数: 0

Abstract

Background

While dental pulp stem cells (DPSCs) show therapeutic potential for spinal cord injury (SCI), post-traumatic oxidative stress and mitochondrial dysfunction critically compromise grafted cell survival. Photobiomodulation therapy (PBMT) is a noninvasive approach that may enhance regenerative efficacy. This study investigates whether PBMT improves the survival and neural differentiation of transplanted DPSCs in SCI models.

Methods

In vitro, the anti-apoptotic effects of PBMT were investigated in H₂O₂-induced DPSCs injury model via TUNEL staining, flow cytometry, biochemical assays and transmission electron microscopy. In vivo, spinal cord restoration was evaluated using behavioral tests and histological staining, and the survival status and neural differentiation of grafted DPSCs were respectively tracked through bioluminescence imaging and immunofluorescent staining in DPSCs co-expressing luciferin and green fluorescent protein (Luc-GFP-DPSCs) transplanted SCI mice with/without PBMT.

Results

PBMT demonstrated protective effects by relieving apoptosis and oxidative stress in engrafted DPSCs in vitro. Furthermore, PBMT-assisted DPSCs transplantation significantly promoted cell survival and neural differentiation, achieving superior functional recovery compared to transplantation alone.

Conclusions

PBMT facilitates DPSCs transplantation in SCI through mitigation of oxidative apoptosis and enhancement of grafted cell survival and differentiation.

查看原文本刊更多论文

光生物调节疗法促进髓质干细胞移植治疗脊髓损伤。

背景：虽然牙髓干细胞（DPSCs）显示出治疗脊髓损伤（SCI）的潜力，但创伤后氧化应激和线粒体功能障碍严重损害了移植细胞的存活。光生物调节疗法（PBMT）是一种可以提高再生疗效的无创方法。本研究探讨PBMT是否能改善脊髓损伤模型中移植DPSCs的存活和神经分化。方法：通过TUNEL染色、流式细胞术、生化及透射电镜观察PBMT对h2o2诱导的DPSCs损伤模型的抗凋亡作用。在体内，通过行为学测试和组织学染色评估脊髓恢复情况，并通过生物发光成像和免疫荧光染色分别追踪移植脊髓损伤小鼠DPSCs共表达荧光素和绿色荧光蛋白（Luc-GFP-DPSCs）移植脊髓损伤小鼠的存活状况和神经分化情况。结果：PBMT通过减轻体外移植的DPSCs的凋亡和氧化应激表现出保护作用。此外，pbmt辅助的DPSCs移植显著促进了细胞存活和神经分化，与单独移植相比，实现了更好的功能恢复。结论：PBMT通过减轻氧化凋亡和增强移植物细胞存活和分化促进脊髓损伤DPSCs移植。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodiagnosis and Photodynamic Therapy ONCOLOGY-

CiteScore

5.80

自引率

24.20%

发文量

509

审稿时长

50 days

期刊介绍： Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.