Apoptosis Inhibitor of Macrophage (CD5-Like Antigen) in Healthy Dogs and Dogs With Acute Kidney Injury

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2025-03-18 DOI:10.1111/jvim.70053

William H. Whitehouse, Rodrigo Mohallem, Uma K. Aryal

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Abstract

Background

Apoptosis inhibitor of macrophage (AIM) is a fundamental mediator of recovery from acute kidney injury (AKI) in mice, but its role in AKI in dogs is unknown.

Hypothesis/Objectives

To detect and quantify AIM in serum and urine from both healthy dogs and dogs with AKI, and to determine if AIM is higher in dogs with AKI compared to healthy controls.

Animals

Eight dogs with International Renal Interest Society (IRIS) Grade II–V AKI and 10 healthy adult dogs.

Methods

Retrospective case–control study. Liquid chromatography-mass spectrometry (LC–MS) based targeted proteomics was used to quantify AIM. AIM peak areas were compared between the AKI and healthy cohorts and correlated with selected markers of renal function.

Results

AIM was able to be quantified in the urine of 5/8 dogs with AKI and 1/10 healthy dogs. AIM was quantified in the serum of all dogs, and there was no difference in peak area between the two groups (AKI: median, 67 840 (range, 9797-98 725); control: median, 79 072 (range, 46 400-160 330); p = 0.274). In dogs with AKI, AIM was not correlated with serum creatinine, blood urea nitrogen, phosphorus, or potassium concentrations, urine specific gravity, or IRIS AKI grade.

Conclusions and Clinical Importance

AIM was predominantly detected in the urine of dogs with AKI and not in the urine of healthy dogs.

Abstract Image

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背景巨噬细胞凋亡抑制因子（AIM）是小鼠急性肾损伤（AKI）恢复的基本介质，但它在犬 AKI 中的作用尚不清楚。假设/目标检测健康犬和 AKI 患犬血清和尿液中的 AIM 并对其进行定量，确定 AKI 患犬的 AIM 是否高于健康对照组。动物 8 只患有国际肾脏兴趣协会 (IRIS) II-V 级 AKI 的狗和 10 只健康成年狗。方法回顾性病例对照研究。采用基于液相色谱-质谱（LC-MS）的靶向蛋白质组学对 AIM 进行定量。比较了 AKI 和健康组群的 AIM 峰面积，并将其与选定的肾功能标记物进行了相关性分析。结果 5/8 只患有 AKI 的狗和 1/10 只健康狗的尿液中都能定量检测到 AIM。所有狗的血清中都能定量检测到 AIM，两组之间的峰面积没有差异（AKI：中位数，67 840（范围，9797-98 725）；对照组：中位数，79 072（范围，46 400-160 330）；P = 0.274）。在患有 AKI 的狗中，AIM 与血清肌酐、血尿素氮、磷或钾浓度、尿比重或 IRIS AKI 分级无关。结论和临床意义 AIM 主要在 AKI 患犬的尿液中检测到，而不在健康犬的尿液中检测到。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.