{"title":"The Effect of Immobilization Method on DNA Stretching Characteristics","authors":"M. A. Didin","doi":"10.1134/S0006350924701070","DOIUrl":null,"url":null,"abstract":"<div><p>The problem of stretching a flexible polymer molecule with specified conditions at the ends under the action of a fixed force is considered. A mathematical model has been constructed for various energy functions describing the elasticity of the polymer and various immobilization methods. It is shown that the dependence of stretching on the length of the polymer is linear. The results of numerical calculations are presented. The results obtained make it possible to improve the analysis of experimental data on polymer stretching. The theory provides several verifiable predictions, including experimental methods that improve the accuracy of measurements.</p></div>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"69 6","pages":"990 - 1002"},"PeriodicalIF":4.0330,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350924701070","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
The problem of stretching a flexible polymer molecule with specified conditions at the ends under the action of a fixed force is considered. A mathematical model has been constructed for various energy functions describing the elasticity of the polymer and various immobilization methods. It is shown that the dependence of stretching on the length of the polymer is linear. The results of numerical calculations are presented. The results obtained make it possible to improve the analysis of experimental data on polymer stretching. The theory provides several verifiable predictions, including experimental methods that improve the accuracy of measurements.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍:
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.