The Effects of Ethidium Bromide on Purinergic Modulation of Myoneural Transmission and Skeletal Muscle Contraction

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2025-03-17 DOI:10.1134/S0006350924701203

A. N. Gorshunova, A. Yu. Teplov, S. N. Grishin, R. D. Mukhamedzyanov, A. E. Khairullin

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Abstract

The problem of changes in myoneural transmission in the presence of an intercalating agent, ethidium bromide, which has a known inhibitory effect on neuromuscular transmission, has been investigated, but the nature of such an effect remains unclear. To solve the question of the possible participation in this process of known modulators of synaptic transmission, that is, purines (ATP and adenosine), we evaluated their effects in the presence of this agent. After holding a frog neuromuscular preparation in a perfusing solution containing ethidium bromide, the amplitude of postsynaptic responses and muscle contraction forces decreased. Under these conditions, both purines additionally exerted their usual suppressive effect on both the amplitude of postsynaptic responses and the strength of skeletal muscle contraction. Thus, the inhibitory effect of ethidium bromide on neuromuscular transmission is not associated with an increase in the inhibitory effect of endogenous purines caused by the quantum release of the neurotransmitter.

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溴化乙锭对嘌呤能调节肌神经递质和骨骼肌收缩的影响

在一种已知对神经肌肉传递有抑制作用的插入剂——溴化乙锭存在时，肌神经传递的变化问题已被研究过，但这种作用的性质仍不清楚。为了解决已知突触传递调节剂嘌呤（ATP和腺苷）可能参与这一过程的问题，我们评估了它们在这种药物存在下的作用。在含有溴化乙锭的灌注溶液中持有蛙神经肌肉制剂后，突触后反应的振幅和肌肉收缩力下降。在这些条件下，这两种嘌呤对突触后反应的幅度和骨骼肌收缩的强度都发挥了它们通常的抑制作用。因此，溴化乙锭对神经肌肉传递的抑制作用与神经递质量子释放引起的内源性嘌呤抑制作用的增加无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.