{"title":"The Quantitative Determination of the Total Antioxidant Activity of Tears","authors":"V. V. Tiulina, I. I. Senin","doi":"10.1134/S1990750824600961","DOIUrl":null,"url":null,"abstract":"<p>In the present study, the authors developed a protocol for determining the total antioxidant activity of tears. For this purpose, a chemiluminescent model of free-radical oxidation of luminol initiated in the presence of hemoglobin and hydrogen peroxide was used. This approach allows for controlling the kinetics of inhibition of luminol oxidation by tears. Total antioxidant activity was evaluated by the induction period, which showed the delay time of the initiation of luminol oxidation. The calibration curve for tear fluid was linear within the range from 0.5 to 7 μL (<i>r</i><sup>2</sup> = 0.972). The advantages of the developed protocol are high sensitivity, reproducibility, and rapidity of the assay. This makes our protocol suitable for determining the total antioxidant activity of tears.\n\n<b>Keywords</b>: total antioxidant activity, tear fluid, antioxidants, chemiluminescence</p>","PeriodicalId":485,"journal":{"name":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","volume":"18 1 supplement","pages":"S18 - S25"},"PeriodicalIF":0.6000,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1134/S1990750824600961","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
In the present study, the authors developed a protocol for determining the total antioxidant activity of tears. For this purpose, a chemiluminescent model of free-radical oxidation of luminol initiated in the presence of hemoglobin and hydrogen peroxide was used. This approach allows for controlling the kinetics of inhibition of luminol oxidation by tears. Total antioxidant activity was evaluated by the induction period, which showed the delay time of the initiation of luminol oxidation. The calibration curve for tear fluid was linear within the range from 0.5 to 7 μL (r2 = 0.972). The advantages of the developed protocol are high sensitivity, reproducibility, and rapidity of the assay. This makes our protocol suitable for determining the total antioxidant activity of tears.
Keywords: total antioxidant activity, tear fluid, antioxidants, chemiluminescence
期刊介绍：
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry covers all major aspects of biomedical chemistry and related areas, including proteomics and molecular biology of (patho)physiological processes, biochemistry, neurochemistry, immunochemistry and clinical chemistry, bioinformatics, gene therapy, drug design and delivery, biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine. The journal also publishes review articles. All issues of the journal usually contain solicited reviews.
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