p-chlorophenylalanine treatment accelerates tomato fruit ripening through hormone synthesis and glycolytic pathway during storage

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2025-03-17 DOI:10.1016/j.postharvbio.2025.113517

Keyu Xu , Ran Yan , Hang Zhang , Lilai Yin , Wenbo Lei , Jianhui Cheng , Chen Huan , Yan Liu , Xiaolin Zheng , Shuling Shen

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引用次数: 0

Abstract

The accumulation of lycopene is the important indicator of tomato ripening and regulated by multiple plant signaling molecules. In this study, the effects of 30 μM p-chlorophenylalanine (p-CPA) on the postharvest ripening associated with lycopene content of tomato fruit were investigated during storage. The results showed that p-CPA treatment accelerated tomato fruit ripening, as indicated by increases in a* value, respiration rate and ethylene production, and decreases in firmness. p-CPA inhibited endogenous melatonin synthesis by competitive binding with tryptamine 5-hydroxylase (T5H), reducing the content of serotonin (the melatonin precursor), and down-regulating the genes expression of SlT5H, SlSNAT and SlASMT, while promoted ethylene synthesis and signal transduction by regulating the related genes. Meanwhile, p-CPA treatment enhanced the glycolytic pathway due to increasing the activity of 6-phosphofructokinase (PFK) and the contents of 3-phosphoglycerolaldehyde and pyruvate compared to the control. Also, p-CPA treatment enhanced the expressions of genes involved in lycopene synthesis including SlPSY1, SlPDS, SlZDS and SlCRTISO, thereby promoting the accumulation of lycopene. Thus, p-CPA treatment promoted tomato ripening along with lycopene accumulation by regulating endogenous melatonin and ethylene contents and activating glycolytic pathway during postharvest.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.