Development and performance evaluation of a magnetic self-assembled electrochemical biosensor based on α-Fe2O3/Fe3O4 heterogeneous nanorods for supersensitive detection of KRAS

IF 4.9 2区化学 Q2 CHEMISTRY, PHYSICAL

Colloids and Surfaces A: Physicochemical and Engineering Aspects Pub Date : 2025-03-15 DOI:10.1016/j.colsurfa.2025.136624

Qingxiang Wang , Hezhong Ouyang , Wentao Li , Hexiao Zhang , Aolin He , Ruijiang Liu

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引用次数: 0

Abstract

The Kirsten Rat Sarcoma Viral Oncogene (KRAS) represents one of the most frequently mutated oncogenes in a variety of malignant tumors, including non-small cell lung cancer (NSCLC), colorectal cancer (CRC), and pancreatic ductal adenocarcinoma (PDAC). Therefore, it is regarded as a pivotal tumor marker, and the detection of it holds immense significance in the realm of tumor prevention, diagnosis, and disease monitoring. In this project, an α-Fe₂O₃/Fe₃O₄@Au-DNA/BSA probe was progressively constructed to capture KRAS, and the binding probe was immobilized onto the surface of magnetic glass carbon electrodes (MGCE) through magnetic self-assembly for detection purposes. The fabrication process was validated by changes observed in cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) data. Differential pulse voltammetry (DPV) was employed to optimize crucial experimental conditions and analyze biosensor performance. This condition optimization resulted in enhanced sensitivity of the biosensor, leading to improved linearity of data obtained. The limit of detection (LOD) achieved was 1.93 pM, with a limit of quantification (LOQ) at 5.84 pM, and a linear range ranging from 10 pM to 1 μM. Additionally, the stability (the current maintaining stability during the initial eight days), reproducibility (RSD = 2.47 %), selectivity, and real sample analysis (recovery rates ranging from 95.26 % to 109.80 %, RSD ≤ 1.22 %) were found to be favorable. These findings suggest promising prospects for practical applications of this biosensor.

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来源期刊

Colloids and Surfaces A: Physicochemical and Engineering Aspects 化学-物理化学

CiteScore

8.70

自引率

9.60%

发文量

2421

审稿时长

56 days

期刊介绍： Colloids and Surfaces A: Physicochemical and Engineering Aspects is an international journal devoted to the science underlying applications of colloids and interfacial phenomena. The journal aims at publishing high quality research papers featuring new materials or new insights into the role of colloid and interface science in (for example) food, energy, minerals processing, pharmaceuticals or the environment.