Bioactive silver nanoparticles derived from Carica papaya floral extract and its dual-functioning biomedical application.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-03-15 DOI:10.1038/s41598-025-93864-y

E S Harsha Haridas, M K Ravi Varma, Goutam Kumar Chandra

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Abstract

Replacing synthetic phytochemicals with natural plant extracts for metal nanoparticle synthesis enable cost-effective, large-scale production with reduced environmental and health risks while enhancing biomedical efficacy. This study presents the green synthesis of silver nanoparticles (AgNPs) using a flavonol-enriched extract from male papaya flowers (KQE), an underutilized agricultural waste. Using 20% (v/v) KQE, highly stable, spherical KQ-AgNPs (12.3 ± 3.0 nm) were synthesized via in-situ generation of free radicals, such as ortho-quinones, which reduced Ag⁺ ions. KQ-AgNPs exhibit superior antibacterial activity against both gram-positive and gram-negative bacteria compared to chemically synthesized AgNPs (AgNPs-Chem) and KQE alone. In vitro anticancer assays reveal enhanced cytotoxicity against breast carcinoma cells (MCF-7) with an IC₅₀ of 21.25 ± 1.14 µg/mL, significantly lower than AgNPs-Chem (33.05 ± 3.13 µg/mL), while maintaining high biocompatibility with normal cells (HEK-293) with a greater IC₅₀ of 169.96 ± 2.3 µg/mL. This study highlights the dual therapeutic potential of KQ-AgNPs, emphasizing their enhanced antibacterial and anticancer efficacy while exemplifying an innovative waste-to-wealth approach.

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从番木瓜花提取物中提取的生物活性纳米银及其双功能生物医学应用。

用天然植物提取物代替合成植物化学物质进行金属纳米颗粒合成，可实现成本效益高的大规模生产，减少环境和健康风险，同时提高生物医学功效。本研究介绍了利用未充分利用的农业废弃物——雄木瓜花（KQE）中富含黄酮醇的提取物绿色合成纳米银颗粒（AgNPs）。在20% (v/v) KQE的条件下，通过原位生成对醌等自由基还原Ag+，合成了高度稳定的球形KQ-AgNPs（12.3±3.0 nm）。与化学合成的AgNPs （AgNPs- chem）和单独的KQE相比，KQ-AgNPs对革兰氏阳性和革兰氏阴性细菌都具有更好的抗菌活性。体外抗癌实验显示，AgNPs-Chem对乳腺癌细胞（MCF-7）的IC50为21.25±1.14µg/mL，显著低于AgNPs-Chem(33.05±3.13µg/mL)，同时与正常细胞（HEK-293）保持较高的生物相容性，IC50为169.96±2.3µg/mL。这项研究强调了KQ-AgNPs的双重治疗潜力，强调了它们增强的抗菌和抗癌功效，同时举例说明了一种创新的废物转化为财富的方法。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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