Invadopodia Related-Proteins Expression in Mucoepidermoid Carcinoma.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-03-14 DOI:10.1111/odi.15312

Felippe José Almeida Loureiro, Karolyny Martins Balbinot, Maria Sueli da Silva Kataoka, Hellen Thais Fuzii, Sérgio de Melo Alves Júnior, José Antônio Picanço Diniz Júnior, Felipe Tuji de Castro Franco, João de Jesus Viana Pinheiro

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引用次数: 0

Abstract

Objective: This study aimed to assess the expression of invadopodia-related proteins in mucoepidermoid carcinoma and their influence on this tumor's invasiveness.

Material and methods: Twenty-seven mucoepidermoid carcinoma grading samples were evaluated for the expression of Tks4, Tks5, cortactin, and MT1-MMP and compared to 10 control samples of normal-looking salivary glands by immunohistochemistry. For in vitro analysis, immunofluorescence identified the expression of invadopodia-related proteins in the mucoepidermoid carcinoma cell line. Invadopodia formation and invasion assays were performed after silencing of Tks4 and Tks5 to evaluate invasiveness.

Results: The invadopodia-related proteins were expressed significantly higher in mucoepidermoid carcinoma samples when compared to the control group. Positive expression of these proteins was identified in the mucoepidermoid carcinoma cell line. Knockdown of Tks4 and Tks5 reduced both gelatin degradation and invadopodia activity in mucoepidermoid carcinoma cell lines.

Conclusion: Our results suggest that mucoepidermoid carcinoma behavior can be mediated by the expression of invadopodia-related proteins. Tks4 and Tks5 play a role in the invasiveness of mucoepidermoid carcinoma, mediated by invadopodia.

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.