Invadopodia Related-Proteins Expression in Mucoepidermoid Carcinoma.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-03-14 DOI:10.1111/odi.15312

Felippe José Almeida Loureiro, Karolyny Martins Balbinot, Maria Sueli da Silva Kataoka, Hellen Thais Fuzii, Sérgio de Melo Alves Júnior, José Antônio Picanço Diniz Júnior, Felipe Tuji de Castro Franco, João de Jesus Viana Pinheiro

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引用次数: 0

Abstract

Objective: This study aimed to assess the expression of invadopodia-related proteins in mucoepidermoid carcinoma and their influence on this tumor's invasiveness.

Material and methods: Twenty-seven mucoepidermoid carcinoma grading samples were evaluated for the expression of Tks4, Tks5, cortactin, and MT1-MMP and compared to 10 control samples of normal-looking salivary glands by immunohistochemistry. For in vitro analysis, immunofluorescence identified the expression of invadopodia-related proteins in the mucoepidermoid carcinoma cell line. Invadopodia formation and invasion assays were performed after silencing of Tks4 and Tks5 to evaluate invasiveness.

Results: The invadopodia-related proteins were expressed significantly higher in mucoepidermoid carcinoma samples when compared to the control group. Positive expression of these proteins was identified in the mucoepidermoid carcinoma cell line. Knockdown of Tks4 and Tks5 reduced both gelatin degradation and invadopodia activity in mucoepidermoid carcinoma cell lines.

Conclusion: Our results suggest that mucoepidermoid carcinoma behavior can be mediated by the expression of invadopodia-related proteins. Tks4 and Tks5 play a role in the invasiveness of mucoepidermoid carcinoma, mediated by invadopodia.

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黏液表皮样癌中浸润相关蛋白的表达。

目的：探讨黏液表皮样癌中浸润相关蛋白的表达及其对肿瘤侵袭性的影响。材料和方法：对27例粘液表皮样癌分级样本进行Tks4、Tks5、cortatin和MT1-MMP的表达评估，并与10例外观正常的唾液腺对照样本进行免疫组织化学比较。在体外分析中，免疫荧光鉴定了黏液表皮样癌细胞系中侵过相关蛋白的表达。在沉默Tks4和Tks5基因后，进行侵入性形成和侵袭性测定，以评估侵袭性。结果：黏液表皮样癌组织中侵过相关蛋白的表达明显高于对照组。这些蛋白在黏液表皮样癌细胞系中表达阳性。敲低Tks4和Tks5可降低黏液表皮样癌细胞的明胶降解和侵入性活性。结论：黏液表皮样癌的行为可能受侵过相关蛋白的表达介导。Tks4和Tks5在黏液表皮样癌的侵袭中发挥作用，由侵足介导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.