The assembly of a Malus sieversii regulatory network reveals gene resistance against Alternaria alternata f. sp. mali when colonized by Trichoderma biofertilizer.

IF 2.7 3区 生物学 Q2 PLANT SCIENCES
Journal of Plant Research Pub Date : 2025-05-01 Epub Date: 2025-03-15 DOI:10.1007/s10265-025-01622-y
Shida Ji, Dechen Li, Jin Yao, Bin Liu, Jing Han, Yucheng Wang, Zhihua Liu
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引用次数: 0

Abstract

Trichoderma spp., as excellent biocontrol agents, can induce systemic resistance to protect plants from phytopathogen attacks. In a previous study, Trichoderma biofertilizer activated the MsERF105 transcription factor (TF), which further enhanced the resistance of Malus sieversii against Alternaria alternata f. sp. mali, but how resistance signals are transmitted is still unknown. In this study, it was found that the MsERF105-centered disease-resistant regulatory network was induced by Trichoderma in M. sieversii. The TF-centered yeast one-hybrid indicated that WRKY33 and WRKY40 bound to WBOXATNPR1 elements and GT1 bound to GT1CONSENSUS elements in the promoter of MsERF105 to activate its expression. In addition, the proteins that interacted with MsERF105 were identified by yeast two-hybrid, including FUBP2 and HSP17.8. Furthermore, the candidate target genes of MsERF105 were screened using RNA-Seq, and yeast one-hybrid and tobacco transient transformation further showed MsERF105 bound to GCCBOX elements to regulate the expression of bHLH162, ERF017, NAC83 and NAC104; bound to CCAATBOX elements to regulate the expression of HSFs, HSP70s and HSP20; and bound to ERS elements to regulate the expression of DRPs. Finally, the Trichoderma-induced MsERF105-centered regulatory network of M. sieversii against A. alternata f. sp. mali was built, which provided reliable theoretical guidance for the application of Trichoderma and the disease-resistance breeding of M. sieversii.

海参调控网络的组装揭示了当木霉生物肥料定殖时对马利交替稻瘟菌的基因抗性。
木霉(Trichoderma spp)作为一种优良的生物防治剂,可诱导植物产生全身抗性,保护植物免受病原菌的侵害。在之前的研究中,木霉生物肥料激活了MsERF105转录因子(TF),进一步增强了Malus siversii对alternnaria alternata f. sp. mali的抗性,但抗性信号是如何传递的尚不清楚。本研究发现西韦氏分枝杆菌中以mserf105为中心的抗病调控网络是由木霉诱导的。以tf为中心的酵母单杂交表明,WRKY33和WRKY40与MsERF105启动子中的WBOXATNPR1元件结合,GT1与GT1 consensus元件结合激活MsERF105的表达。此外,通过酵母双杂交鉴定了与MsERF105相互作用的蛋白,包括FUBP2和HSP17.8。利用RNA-Seq筛选MsERF105的候选靶基因,通过酵母单杂交和烟草瞬时转化进一步发现,MsERF105与GCCBOX元件结合,调控bHLH162、ERF017、NAC83和NAC104的表达;与CCAATBOX元件结合,调控hsf、hsp70和HSP20的表达;并与ERS元件结合,调控DRPs的表达。最后,构建了以木霉诱导的以mserf105为中心的西韦氏分枝杆菌对麻枯草芽孢杆菌的调控网络,为木霉的应用和西韦氏分枝杆菌的抗病育种提供了可靠的理论指导。
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来源期刊
Journal of Plant Research
Journal of Plant Research 生物-植物科学
CiteScore
5.40
自引率
3.60%
发文量
59
审稿时长
1 months
期刊介绍: The Journal of Plant Research is an international publication that gathers and disseminates fundamental knowledge in all areas of plant sciences. Coverage extends to every corner of the field, including such topics as evolutionary biology, phylogeography, phylogeny, taxonomy, genetics, ecology, morphology, physiology, developmental biology, cell biology, molecular biology, biochemistry, biophysics, bioinformatics, and systems biology. The journal presents full-length research articles that describe original and fundamental findings of significance that contribute to understanding of plants, as well as shorter communications reporting significant new findings, technical notes on new methodology, and invited review articles.
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