Cryoprotective effect of zinc and gold nanoparticles during cooling and freeze-thawing on Marwari stallion sperm parameters and reactive oxygen species production.

IF 0.9 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-11-01

T Sultan, A K Chaudhary, J S Mehta, R Mehra, A Manuja, T R Talluri, T K Bhattacharya

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引用次数: 0

Abstract

Background: Sperm cryopreservation is one of the most important procedures in the development of biotechnologies for assisted reproduction. Cryopreservation of stallion spermatozoa tends to cause plasma membrane damage due to the low ratio of cholesterol to phospholipids. Different substances and compounds can be added to semen extenders to improve sperm quality.

Objective: To investigate the effect of supplementing semen extender with zinc nanoparticles (ZnONPs) and gold nanoparticles (AuNPs) on cooled and frozen-thawed spermatozoa of Marwari stallion.

Materials and methods: A total of 20 ejaculates from four Marwari stallions (five ejaculates from each) were collected. The gel free semen was extended with primary extender in equal volume and then divided in three equal groups, namely control (C), zinc (ZnO) and gold (Au), and centrifuged to obtain a final concentration of 100-150 x10⁶ mL^-1 and then cryopreserved. Cooled and post-thaw semen evaluations were conducted for various seminal characteristics, viz. progressive sperm motility, sperm plasma membrane integrity, sperm viability, acrosome integrity and mitochondrial membrane potential.

Results: Cooled semen (4 degree C for 2 h) evaluation revealed non-significant differences among the groups (C, ZnO and Au) for all the semen quality parameters. However, at the post-thaw stage, progressive sperm motility, sperm plasma membrane integrity, acrosome integrity and oxidative parameters were significantly (P < 0.01) higher in the ZnO group than Au and C.

Conclusion: The addition of ZnO nanoparticles improves the post thaw quality of stallion spermatozoa by reducing the oxidative stress, but Au nanoparticles had no effect on cooled as well as post-thaw semen quality. https://doi.org/10.54680/fr24610110212.

本刊更多论文

锌和金纳米颗粒在冷却和冻融过程中对马尔瓦里种马精子参数和活性氧产生的冷冻保护作用。

背景：精子冷冻保存是辅助生殖生物技术发展中最重要的技术之一。由于种马精子中胆固醇与磷脂的比例较低，低温保存容易造成质膜损伤。可以在精液填充剂中添加不同的物质和化合物来提高精子质量。目的：研究在精液补充剂中添加纳米锌粒子（ZnONPs）和纳米金粒子（AuNPs）对马尔瓦里种马冷冻和冻融精子的影响。材料与方法：收集4匹马尔瓦里种马20次射精（每匹5次）。将无凝胶精液用原填充剂等量拉伸后，分成对照(C)、锌（ZnO）、金（Au）等3组，离心至终浓度100-150 x106 mL-1，冷冻保存。对冷却和解冻后的精液进行各种精液特性的评估，包括精子运动能力、精子质膜完整性、精子活力、顶体完整性和线粒体膜电位。结果：冷却精液（4度C， 2小时）评价显示，C、ZnO和Au组之间所有精液质量参数均无显著差异。但在解冻后阶段，氧化锌组的精子活力、精子质膜完整性、顶体完整性和氧化参数均显著（P < 0.01）高于Au组和c组。结论：氧化锌纳米粒子通过降低氧化应激提高了解冻后种马精子的质量，而Au纳米粒子对解冻后精子的冷却和解冻后精液质量没有影响。https://doi.org/10.54680/fr24610110212。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.