Effect of Hericium erinaceus polysaccharides on the cryopreservation of bull semen.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-03-01

M I Sergushkina, T V Polezhaeva, O N Solomina, A N Khudyakov, O O Zaitseva, Y I Nazarova

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引用次数: 0

Abstract

Background: The field of sperm cryopreservation requires the search for and development of new effective cryopreservatives with low toxicity.

Objective: The purpose of this study was to evaluate the characteristics of bull spermatozoa subjected to freezing and storage at -80 degree C for 28 days in a diluent with the addition of Hericium erinaceus polysaccharides.

Materials and methods: The sperm was frozen under the protection of a complex cryoprotectant and stored in an electric freezer at -80 degree C for 28 days. Before cooling and after defrosting, the biological parameters of the sperm were analyzed using the Argus-CASA system.

Results: It was established that two fractions isolated from H. erinaceus (PF1, PF2) are heteropolysaccharide peptides with a high content of glucose, xylose, mannose, galactose and arabinose. It was shown that before cooling, when a fraction obtained from dry fruiting bodies of H. erinaceus (PF2) was added to the sperm, the number of progressively motile sperm with intact DNA decreased, but their kinematic parameters did not change. After thawing, after 28 days of storage at -80 degree C, an increase in the number of progressively motile sperm with intact DNA and their kinematic parameters was observed.

Conclusion: Thus, fungal polysaccharides are obviously underestimated macromolecular substances with enormous cryostabilization potential, and polysaccharide fractions from dried fruiting bodies of H. erinaceus may in the future find wide application in the composition of new cryopreservation media. https://doi.org/10.54680/fr25210110912.

本刊更多论文

猴头菌多糖对牛精液冷冻保存的影响。

背景：精子低温保存领域需要寻找和开发新的低毒性、有效的低温保鲜剂。目的：研究牛精在添加猴头菌多糖的稀释液中-80℃冷冻保存28 d后的性状。材料和方法：精子在复合冷冻保护剂的保护下冷冻，在-80℃的电冰箱中保存28天。在冷却前和解冻后，使用Argus-CASA系统分析精子的生物学参数。结果：从羊角草中分离得到的两个部位（PF1、PF2）均为含葡萄糖、木糖、甘露糖、半乳糖和阿拉伯糖的多糖多肽。结果表明，在冷却前，在精子中加入一种从erinaceus干子实体中提取的部分（PF2）， DNA完整的精子数量逐渐减少，但其运动参数没有改变。解冻后，在-80℃下储存28天后，观察到DNA完好无损的精子数量和运动学参数逐渐增加。结论：真菌多糖是一种被明显低估的大分子物质，具有巨大的冷冻稳定潜力，今后在制备新型冷冻保存介质中有广阔的应用前景。https://doi.org/10.54680/fr25210110912。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.