Effects of cryolipolysis on subcutaneous adipose tissue of adult women: immunohistochemical analysis.

IF 0.9 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2025-01-01

C Rt Palauro, P S Palmeira Silva Daumas, E M Carreiro, F Pimentel de Almeida, I L Dias, P F Meyer

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引用次数: 0

Abstract

Background: The skin, the largest organ in the human body, is composed of complex layers that include subcutaneous adipose tissue. Understanding the characteristics of this skin structure is essential to optimize therapeutic interventions, such as cryolipolysis, aiming for more effective and personalized results.

Objective: To evaluate the immunohistochemical effects of skin tissue in adult women undergoing cryolipolysis.

Materials and methods: We carried out an experimental and blind study with immunohistochemical analysis in women with localized abdominal fat, categorized based on the constitution of the skin as flaccid or firm according to the Investigator Assessment Skin Laxity Scoring System scale. Participants were randomized before undergoing the cryolipolysis procedure. Forty-five days after the procedure, they underwent abdominoplasty, with collection of biological material. We evaluated the inflammatory markers EBF-1, TNF-alpha, and CD68, as well as Caspase 3, cleaved Caspase 3, apoptotic BCL2, Ki-67 for fibroblast proliferation, and FIS1 for mitochondrial proliferation.

Results: Six women were included, divided into two groups; three women with loose and three with firm skin. We observed that after cryolipolysis, the group with flaccid skin showed higher expression of the Casp3, TNF-alpha, BCL2, and FIS1 markers compared to those with firm skin.

Conclusion: Cryolipolysis may act differently according to tissue morphology, suggesting that its apoptotic response is more pronounced in the group with flaccid skin. https://doi.org/10.54680/fr25110110212.

本刊更多论文

冷冻脂肪分解对成年女性皮下脂肪组织的影响：免疫组织化学分析。

背景：皮肤是人体最大的器官，由包括皮下脂肪组织在内的复杂层组成。了解这种皮肤结构的特征对于优化治疗干预（如冷冻脂肪溶解）至关重要，旨在获得更有效和个性化的结果。目的：探讨冷冻脂肪溶解术对成年女性皮肤组织的免疫组化影响。材料和方法：我们对局部腹部脂肪的女性进行了一项实验和盲法免疫组织化学分析研究，根据研究者评估皮肤松弛评分系统量表，根据皮肤构成分为松弛或紧致。参与者在接受冷冻脂肪溶解手术前被随机化。手术45天后，他们接受了腹部成形术，收集了生物材料。我们评估了炎症标志物EBF-1、tnf - α和CD68，以及Caspase 3、cleaved Caspase 3、凋亡的BCL2、Ki-67（成纤维细胞增殖）和FIS1（线粒体增殖）。结果：纳入6名女性，分为两组；三个皮肤松弛的，三个皮肤紧实的。我们观察到，在冷冻脂肪分解后，皮肤松弛组与皮肤紧致组相比，Casp3、tnf - α、BCL2和FIS1标志物的表达更高。结论：冷冻脂肪溶解作用可能因组织形态不同而不同，提示其凋亡反应在皮肤松弛组更为明显。https://doi.org/10.54680/fr25110110212。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.