Bovine coronavirus presence in domestic bovine and antelopes sub-Saharan Africa: evidence from Namibia.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Umberto Molini, Lauren M Coetzee, Maria Y Hemberger, Mark Jago, Siegfried Khaiseb, Kalihulu Shapwa, Alessio Lorusso, Giovanni Cattoli, William G Dundon, Giovanni Franzo
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Abstract

Background: Bovine coronavirus (BoCV) causes significant economic losses to cattle farming due to mortality in calves, reduced growth performances and milk production in feedlots and dairy cattle. Worldwide distribution of BoCV has been demonstrated, although knowledge of its epidemiology in Africa, especially in the sub-Saharan region, is limited.

Results: In the present study, a total of 208 swab samples of wild ruminants and 435 bovines from different regions of Namibia were obtained and tested by a BoCV-specific qRT-PCR. Twenty-six bovine samples tested positive [26/435 (5.98%; 95CI: 3.94-8.64%)] while, among the wild ruminants, only Greater Kudu (Tragelaphus strepsiceros) were shown to be positive [13/52 (25.00%; 95CI: 14.03-38.95%)] of which 8 showed clinical signs. Analysis of partial nucleoprotein and spike protein gene sequences and comparison with international reference sequences demonstrated the existence of a unique Namibian clade, resulting from a single introduction event around 2010 followed by local evolution. Although the introduction source remains unknown, contact between bovine and wild animals appears likely.

Conclusions: The present study represents the first report of BoCV circulation in southern Africa, which showed a relatively high frequency and the ability of persisting and evolving locally in the absence of further foreign introductions. The implications for disease spread among domestic bovines and the potential impact on wildlife should encourage broader investigations on BoCV involving other African countries. Moreover, the Greater Kudu's susceptibility to BoCV infection was also proven, further highlighting the host plasticity of this virus.

撒哈拉以南非洲家牛和羚羊中存在的牛冠状病毒:纳米比亚的证据。
背景:牛冠状病毒(BoCV)导致犊牛死亡、生长性能下降、饲养场和奶牛产奶量下降,给养牛业造成重大经济损失。尽管对其在非洲,特别是撒哈拉以南地区的流行病学了解有限,但已证实BoCV在世界范围内分布。结果:本研究共获得了来自纳米比亚不同地区的野生反刍动物和435头牛的208份拭子样本,并通过bocv特异性qRT-PCR进行了检测。26份牛样本检测呈阳性[26/435 (5.98%;(95CI: 3.94-8.64%)]而在野生反刍动物中,只有大羚羊(Tragelaphus strepsiceros)呈阳性[13/52 (25.00%;[95CI: 14.03-38.95%],其中8例出现临床症状。对部分核蛋白和刺突蛋白基因序列的分析以及与国际参考序列的比较表明,由于2010年前后的一次引入事件以及随后的局部进化,存在一种独特的纳米比亚进化支。虽然引入源尚不清楚,但牛与野生动物之间的接触似乎是可能的。结论:本研究是南部非洲首次报告的BoCV传播,在没有进一步外来引入的情况下,该传播频率相对较高,并且能够在当地持续和发展。对家畜之间疾病传播的影响以及对野生动物的潜在影响应鼓励在其他非洲国家开展更广泛的BoCV调查。此外,还证实了大库杜对BoCV感染的易感性,进一步突出了该病毒的宿主可塑性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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