Echinocystic acid activates PPARγ to alleviate mannan-induced psoriasis and psoriatic arthritis in mice.

IF 2.5 4区医学 Q3 ALLERGY

Allergologia et immunopathologia Pub Date : 2025-03-01 eCollection Date: 2025-01-01 DOI:10.15586/aei.v53i2.1283

Chengwei Yu, Huiming Wu, Dongrui Zhao, Huajie Shi

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引用次数: 0

Abstract

Previous studies have shown that echinocystic acid (EA) can reduce arthritis and skin damage, but the role of EA in psoriatic arthritis is unclear. This study aims to prove the role of EA in psoriatic arthritis, which was induced by intraperitoneal injection of mannan in C57BL/6J mice. The mice were divided into a control group, mannan group, mannan + EA (low-dose) group, and mannan + EA (high-dose) group. Joint tissue damage was scored, and pathological changes in joint tissue and ear skin damage were examined by HE staining. Pathway enrichment of EA drug targets was performed through the target enrichment website, and the mRNA and protein expression levels of pathway-related proteins in joint tissues and ears were verified using the PCR and western blot. The results show that injection of mannan into mice resulted in joint inflammatory infiltration and tissue damage, hyperkeratosis, and acanthosis of the ear skin, while these symptoms were alleviated after high-dose EA treatment. Pathway enrichment analysis showed that the EA drug treatment target is concentrated on the PPAR pathway. The mRNA and protein results showed that the mRNA and protein expression levels of peroxisome proliferator-activated receptor γ (PPARγ) in the joint tissues and ears of mice with psoriatic arthritis decreased, and the expression of PPARγ was activated after high-dose EA treatment. In conclusion, EA increases PPARγ expression and reduces joint and skin damage in mice with psoriatic arthritis.

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棘囊酸激活PPARγ减轻甘露聚糖诱导的小鼠银屑病和银屑病关节炎。

既往研究表明棘囊酸（EA）可减轻关节炎和皮肤损伤，但EA在银屑病关节炎中的作用尚不清楚。本研究旨在证明EA在C57BL/6J小鼠腹腔注射甘露聚糖诱导的银屑病关节炎中的作用。将小鼠分为对照组、甘露聚糖组、甘露聚糖+ EA（低剂量）组和甘露聚糖+ EA（高剂量）组。对关节组织损伤进行评分，并用HE染色观察关节组织及耳部皮肤损伤的病理变化。通过靶富集网站对EA药物靶点进行通路富集，利用PCR和western blot验证关节组织和耳朵中通路相关蛋白的mRNA和蛋白表达水平。结果表明，甘露聚糖注射小鼠可引起关节炎性浸润及组织损伤、角化过度、耳皮棘层增生，而经大剂量EA治疗后，这些症状均得到缓解。途径富集分析表明，EA药物治疗靶点集中在PPAR通路上。mRNA和蛋白表达结果显示，银屑病关节炎小鼠关节组织和耳部过氧化物酶体增殖物激活受体γ (PPARγ) mRNA和蛋白表达水平降低，且高剂量EA处理后PPARγ表达被激活。综上所述，EA可增加银屑病关节炎小鼠的PPARγ表达，减轻关节和皮肤损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.