Chronic ammonia exposure impairs the growth and intestinal function in juvenile Megalobrama amblycephala

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-03-17 DOI:10.1007/s10499-025-01896-y

Xiangyang Yuan, Han Zhang, Qian Wang, Xinyu Xiong, Mengyang Dai, Wenhui Ye, Yifan Wang, Canli Wang

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引用次数: 0

Abstract

This study investigated the effects of chronic ammonia exposure on the growth parameters, intestinal histology, and digestive enzyme activity of aquatic fish. Blunt snout bream, Megalobrama amblycephala juveniles, with 6.55 ± 0.24 g were exposed to 0 (G1), 6.82 (G2), and 13.64 mg/L (G3) NH₄Cl (ammonium chloride) for 56 days. Then, the intestinal enzyme activity and histological characteristics of blunt snout bream were analyzed using modern biological techniques. The results showed that chronic ammonia exposure significantly decreased the final body weight and specific growth rate of blunt snout bream (p < 0.05). The intestinal protease, lipase, amylase, CK, AKP, and γ-GT activities of blunt snout bream after chronic ammonia exposure significantly decreased (p < 0.05). Meanwhile, the intestinal muscularis thickness and microvillus density of fish decreased as the increased ammonia concentration. The intestinal microvillus length of blunt snout bream after chronic ammonia exposure significantly reduced compared to the control group (p < 0.05). Furthermore, chronic ammonia exposure caused a significant down-regulated expression of Occludin, ZO-1, Claudin-3c, and Claudin-7 mRNA in the intestine (p < 0.05) but up-regulated the levels of NF-κB, TNF-α, IL-1β, and IL-6 mRNA (p < 0.05). Overall, chronic ammonia exposure inhibited the growth and compromised the intestinal structure of fish.

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.