Fertility preservation of vacuum-dried ram spermatozoa stored for four years at room temperature

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-03-13 DOI:10.1016/j.theriogenology.2025.117390

Margherita Moncada , Martina Lo Sterzo , Luca Palazzese , Francesca Boffa , Debora Agata Anzalone , Domenico Iuso , Marta Czernik , Luisa Gioia , Federica Turri , Beatrice Mislei , Diego Bucci , Jacques Bonnet , Marthe Colotte , Sophie Tuffet , Michał Bochenek , Pasqualino Loi

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引用次数: 0

Abstract

Dry storage at room temperature (RT) could simplify spermatozoa banking. Here, we explored DNA stability and in vitro and in vivo development of embryos derived from vacuum-dried encapsulated (VDE) ram spermatozoa stored for four years or after accelerated aging. While some genomic damage was detected at time 0, DNA fragmentation increased from 3.32 ± 3 % (time 0) to 37.64 ± 4 % (4 years). A decrease in blastocyst rate was observed after four years of storage and 6.7 years of simulated storage (10.2 % and 9 % versus 13.16 % at time 0). Embryo quality, assessed based on Cdx2 and Inf-τ gene expression, declined over time. Only two of the 23 embryos transferred into synchronized ewes were implanted but were lost by day 40.

In conclusion, dry spermatozoa generated blastocysts after four years of RT storage, but their post-implantation development was impaired. Optimization of the water extraction and storage conditions could better preserve the spermatozoa's DNA integrity, resulting in improved embryo quality, compatible with development to term.

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常温下真空干燥公羊精子保存4年生育能力的研究

室温干燥储存可以简化精子库。本研究探讨了真空干燥包封（VDE）公羊精子保存4年或加速老化后胚胎的DNA稳定性和体外、体内发育情况。虽然在时间0时检测到一些基因组损伤，但DNA断裂率从3.32±3%（时间0）增加到37.64±4%（4年）。在4年的储存和6.7年的模拟储存后，囊胚率下降（10.2%和9%，而第0次为13.16%）。基于Cdx2和Inf-τ基因表达评估的胚胎质量随着时间的推移而下降。移植到同步母羊体内的23个胚胎中，只有2个被植入，但在第40天就丢失了。总之，干精子在RT储存四年后产生囊胚，但其着床后发育受到损害。优化水分提取和储存条件，可以更好地保存精子DNA的完整性，提高胚胎质量，与发育至足月相适应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.