Experimental Mineralisation of a Filamentous Hydrogenotrophic Methanogen in Carbonate, Phosphate, and Silicate

Abstract

Methanogenic archaea were likely among the earliest organisms to populate the Earth, perhaps contributing to the Archaean greenhouse effect; they are also widely discussed as analogues to any potential life on Mars. However, fossil evidence of archaea has been difficult to identify in the rock record, perhaps because their preservation potential is intrinsically low or because they are particularly small and difficult to identify. Here, we examined the preservation potential of a methanogen of the genus Methanobacterium, recently isolated from a low-temperature serpentinizing system, an environment somewhat analogous to habitats on the early Earth and Mars. Notably, this organism has a cell wall composed of peptidoglycan-like pseudomurein, which may imply a mineralisation potential similar to that of gram-positive bacteria. Methanobacterium cells were placed in carbonate, phosphate, and silicate solutions for up to 3 months in order to assess the relative tendency of these minerals to encrust and preserve cellular morphology. Cells readily acquired a thick, uniform coating of silica, enhancing their potential for long-term preservation while also increasing overall filament size, an effect that may aid the discovery of fossil archaea while hindering their interpretation. Phosphates precipitated from the medium in all experimental setups and even in parallel experiments set up with low-phosphate medium, suggesting a hitherto unknown biomineralisation capacity of methanogens. Carbonate precipitates did not form in close association with cells.