The relationship between microRNA-155-5p and postoperative inflammatory markers in children with acute suppurative appendicitis and its role in predicting postoperative complications.

Chunsheng Duan, Wei Lu, Jiansu Ma, Yanglin Song, Lixia Zhou
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Abstract

Background: The prevalence of acute suppurative appendicitis (ASA) is the highest among pediatric cases of acute abdominal conditions. This research examined the stress response linked to surgical techniques and identified potential biomarkers that could predict postoperative complications to enhance clinical treatment strategies.

Methods: This study involved a selection of 166 ASA patients who underwent laparoscopic appendectomy (LA), and 150 patients who underwent open appendectomy (OA), based on data collected from 2020 to 2023. Comprehensive documentation of clinical and pathological characteristics, as well as postoperative complications, was conducted following patient enrollment. Quantitative polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA), and blood smear techniques were employed to assess the levels of microRNA (miR)-155-5p, C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), superoxide dismutase (SOD), and malondialdehyde (MDA), as well as changes in leukocytes, neutrophils, and lymphocytes at preoperative and postoperative 0 h, and 24 h. A logistic regression model was utilized to identify risk factors associated with the development of complications. Furthermore, receiver operating characteristic (ROC) curve analysis was performed to assess the predictive capacity of miR-155-5p for the occurrence of complications.

Results: The study revealed that the levels of miR-155-5p postoperatively in patients undergoing OA was significantly greater than that observed in patients undergoing LA (P.

急性化脓性阑尾炎患儿microRNA-155-5p与术后炎症标志物的关系及其对术后并发症的预测作用
背景:急性化脓性阑尾炎(ASA)的患病率是儿科急性腹部疾病中最高的。本研究检查了与手术技术相关的应激反应,并确定了可以预测术后并发症的潜在生物标志物,以加强临床治疗策略。方法:本研究选取了166例接受腹腔镜阑尾切除术(LA)的ASA患者和150例接受开放式阑尾切除术(OA)的患者,数据收集于2020年至2023年。在患者入组后,对临床和病理特征以及术后并发症进行了全面的记录。采用定量聚合酶链反应(qPCR)、酶联免疫吸附试验(ELISA)和血液涂片技术,评估术前和术后0 h的microRNA (miR)-155-5p、c反应蛋白(CRP)、白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平以及白细胞、中性粒细胞和淋巴细胞的变化。采用logistic回归模型确定与并发症发生相关的危险因素。进一步进行受试者工作特征(ROC)曲线分析,评估miR-155-5p对并发症发生的预测能力。结果:研究发现OA患者术后miR-155-5p水平明显高于LA患者(P。
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