Prednisolone at a therapeutic dose is not detrimental to mouse oocyte competence.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Shruthivishali Muthukumar, Vijeta Shetty, Akshatha Daddangadi, Satish Kumar Adiga, Shubhashree Uppangala
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引用次数: 0

Abstract

Objective: To examine the impact of PRDL on mouse oocyte developmental competence.

Methods: This study was conducted on 6-8-week-old female Swiss albino mice in which different doses of PRDL were administered for three days during superovulation. Oocytes were assessed for quality, and subsequently, embryos derived from these oocytes were evaluated at the blastocyst stage. Simultaneously, oocytes were also exposed to PRDL during in vitro maturation and examined for their spindle and activation potential.

Results: Compared to control group, the total cell number of blastocysts in the PRDL group increased. Nevertheless, the incidence of apoptosis was comparable between the groups. However, direct exposure at 10 µM significantly increased spindle abnormalities compared to those in the control group (p<0.05).

Conclusions: Short-term PRDL exposure at a therapeutic dose is not detrimental to oocyte developmental potential in vitro. Although the current study confirmed the safety of PRDL in a mouse model, further studies involving long-term exposure are warranted, as patients receive PRDL treatment for extended periods of time.

治疗剂量的强的松龙对小鼠卵母细胞的能力没有损害。
目的:探讨PRDL对小鼠卵母细胞发育能力的影响。方法:本研究采用6-8周龄瑞士白化雌性小鼠,在超排卵期给予不同剂量的PRDL 3天。评估卵母细胞的质量,随后在囊胚期评估这些卵母细胞衍生的胚胎。同时,卵母细胞在体外成熟过程中也暴露于PRDL,并检测其纺锤体和激活电位。结果:与对照组相比,PRDL组囊胚总细胞数增加。然而,两组之间的细胞凋亡发生率是相似的。然而,与对照组相比,直接暴露于10µM的PRDL显著增加了纺锤体异常(结论:短期暴露于治疗剂量的PRDL对体外卵母细胞发育潜能没有损害。虽然目前的研究在小鼠模型中证实了PRDL的安全性,但由于患者接受PRDL治疗的时间较长,因此需要进行涉及长期暴露的进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
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