Genetic insights into Staphylococcus aureus resistance: exploring AMR genes and molecular interactions.

Abstract

Antimicrobial resistance (AMR) among microorganisms remains a significant global concern in this century, posing an ongoing challenge for humanity. To solve this issue effectively, it is crucial to understand the genes responsible for AMR and how they create resistance. Staphylococcus aureus, which has AMR genes imparting resistance against numerous antibiotics, was the main subject of our investigation. We conducted a phylogenetic investigation to explore the evolutionary history of the gene network comprising rpl, rpoC, parE, and gyrB, providing insights into their genetic relationships and evolutionary connections. A gene interaction network with 46 functional partners was built and examined from the STRING Database and Cytoscape to increase our understanding. According to Cluego's enrichment analysis, 20 genes are significantly involved in biological processes, as are 14 genes in cellular components and 16 genes in molecular functions. RpoB, RpoC, FusA, RplI, and RpsL had the most interactions by Cytohubba when the degree and closeness of the network were studied, according to the gene interaction network analysis. Understanding the molecular basis of AMR requires analysis of the enriched pathways and Gene Ontologies (GO). The proposed study may also help researchers find new ways to battle the multidrug resistance of Staphylococcus aureus.