{"title":"Analysis and characteristics of coronaridine, an alkaloid found in <i>Catharanthus roseus</i>.","authors":"Hiroaki Kisaka, Sachise Karakawa, Tetsuya Miwa, Hiroto Hirano, Takashi Onuki, Mayu Iyo","doi":"10.5511/plantbiotechnology.24.0717a","DOIUrl":null,"url":null,"abstract":"<p><p>Coronaridine, a monoterpenoid indole alkaloid, is present in <i>Tabernanthe iboga</i> and the related species <i>Tabernaemontana divaricata</i>. Recent exhaustive analysis revealed its presence in <i>Catharanthus roseus</i>, though specific details remain unknown. We conducted a detailed analysis of coronaridine in <i>C. roseus</i>, detecting it in seedlings post-germination up to 8 weeks after sowing, with peak abundance at 3-4 weeks. Gradual decrease occurred from the flowering stage, and it was absent during seed formation. The accumulation varied dramatically with the plant's growth phase. LC-MS/MS analysis confirmed (-) coronaridine, consistent with <i>T. iboga</i>. Additionally, cultivating at 35°C increased coronaridine accumulation over 10-fold. These findings hold potential for enhancing the stable production of iboga alkaloids for pharmaceutical use.</p>","PeriodicalId":20411,"journal":{"name":"Plant Biotechnology","volume":"41 4","pages":"387-392"},"PeriodicalIF":1.4000,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897734/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.5511/plantbiotechnology.24.0717a","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Coronaridine, a monoterpenoid indole alkaloid, is present in Tabernanthe iboga and the related species Tabernaemontana divaricata. Recent exhaustive analysis revealed its presence in Catharanthus roseus, though specific details remain unknown. We conducted a detailed analysis of coronaridine in C. roseus, detecting it in seedlings post-germination up to 8 weeks after sowing, with peak abundance at 3-4 weeks. Gradual decrease occurred from the flowering stage, and it was absent during seed formation. The accumulation varied dramatically with the plant's growth phase. LC-MS/MS analysis confirmed (-) coronaridine, consistent with T. iboga. Additionally, cultivating at 35°C increased coronaridine accumulation over 10-fold. These findings hold potential for enhancing the stable production of iboga alkaloids for pharmaceutical use.
期刊介绍：
Plant Biotechnology is an international, open-access, and online journal, published every three months by the Japanese Society for Plant Biotechnology. The journal, first published in 1984 as the predecessor journal, “Plant Tissue Culture Letters” and became its present form in 1997 when the society name was renamed to Japanese Society for Plant Cell and Molecular Biology, publishes findings in the areas from basic- to application research of plant biotechnology. The aim of Plant Biotechnology is to publish original and high-impact papers, in the most rapid turnaround time for reviewing, on the plant biotechnology including tissue culture, production of specialized metabolites, transgenic technology, and genome editing technology, and also on the related research fields including molecular biology, cell biology, genetics, plant breeding, plant physiology and biochemistry, metabolic engineering, synthetic biology, and bioinformatics.
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