Capacity to form common mycorrhizal networks reduces the positive impact of clonal integration between plants.

Abstract

Both clonal plant capabilities for physiological integration and common mycorrhizal networks (CMNs) formed by arbuscular mycorrhizal fungi (AMF) can influence the distribution of nutrients and growth among interconnected individuals. Using a microcosm model system, we aimed to disentangle how CMNs interact with clonal integration to influence plant growth and development. We grew Sphagneticola trilobata clones with isolated root systems in individual, adjacent containers while preventing, disrupting, or allowing clonal integration aboveground via spacers and belowground CMNs to form. We assessed multiple metrics of plant development (e.g., growth, specific leaf area, soluble sugar content), ¹⁵N transfer from donor (mother) to receiver (daughter) plants, and variation in AMF communities. We show that spacer formation between ramets and the capacity to form CMNs promoted and inhibited the growth of smaller daughter plants, respectively. In contrast to the independent effects of CMNs and spacers, CMNs, in combination with spacers, significantly weakened the promotion of daughter plants by clonal integration. AMF species richness was also negatively correlated with overall plant growth. Our results demonstrate that two common modes of plant interconnection interact in non-additive ways to affect clonal plant integration and growth. These findings, based on Sphagneticola trilobata, question the underlying assumptions of the positive effects of both AMF CMNs and species richness in comparison to direct plant interconnections.