Extracellular matrix dynamics in a rat model of pulmonary arterial hypertension: unveiling the role of hyaluronan in disease pathology.

IF 3.3 2区 医学 Q1 PERIPHERAL VASCULAR DISEASE
Journal of Hypertension Pub Date : 2025-05-01 Epub Date: 2025-01-16 DOI:10.1097/HJH.0000000000003967
Pinar Altin Çelik, Metin Aytekin
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引用次数: 0

Abstract

Objective: We aimed to investigate the feasibility of conducting extracellular matrix studies within this rat model.

Materials and methods: This study involved 24 Sprague Dawley rats, divided into two groups. Group 1 served as the normoxia control, while Group 2 is SU-5416 pulmonary arterial hypertension (PAH) model. Pulmonary artery pressure (PAP) was measured in both groups using a micro-catheter Power Lab device. Hyaluronan (HA) plasma level was quantified through ELISA, and HA levels were determined via lung tissue immunostaining. Western blotting detected protein levels, and real-time RT-PCR assessed mRNA expressions for HAS1, HAS2, and HAS3 and hyal1 and hyal2.

Results: HA plasma levels were markedly higher in PAH rats compared to controls (HA ng/ml, mean ± SD: PAH 3.8 ± 0.41, control 1.96 ± 0.31, P  < 0.0015). Protein analysis showed no detection of HAS1 and HAS3 proteins in both groups, while HAS2 protein expression was notably higher in PAH rats than controls (HAS2 levels, mean ± SD: PAH 2.0 ± 0.5, control 0.86 ± 0.07, P  = 0.0493). Hyal2 protein expression remained consistent between groups (Hyal2 levels, mean ± SD: PAH 1.41 ± 0.18, control 1.05 ± 0.14, P  = 0.214). No mRNA expression of HAS1, HAS3, and Hyal1 were detected in both groups, Hyal2 expression was identified in both without differences. HAS2 mRNA expression was present in both groups, with a significantly higher increase observed in the hypoxia SU-5416 PAH rat model compared to controls.

Conclusion: Establishing an extracellular matrix profile in the rat model, resembling human PAH under hypoxia SU-5416 conditions, highlights the model's suitability for matrix studies.

肺动脉高压大鼠模型的细胞外基质动力学:揭示透明质酸在疾病病理中的作用。
目的:探讨在该大鼠模型中进行细胞外基质研究的可行性。材料与方法:选取24只Sprague Dawley大鼠,分为两组。1组为常氧对照组,2组为SU-5416肺动脉高压(PAH)模型。两组均采用微导管Power Lab装置测量肺动脉压(PAP)。ELISA法测定血浆透明质酸(HA)水平,肺组织免疫染色法测定HA水平。Western blotting检测蛋白水平,real-time RT-PCR检测HAS1、HAS2、HAS3、hyal1、hyal2 mRNA表达。结果:PAH大鼠血浆HA水平明显高于对照组(HA ng/ml,平均值±SD: PAH 3.8±0.41,对照组1.96±0.31,P)。结论:在大鼠模型中建立细胞外基质谱,与缺氧SU-5416条件下的人PAH相似,表明该模型适合于基质研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Hypertension
Journal of Hypertension 医学-外周血管病
CiteScore
7.90
自引率
6.10%
发文量
1389
审稿时长
3 months
期刊介绍: The Journal of Hypertension publishes papers reporting original clinical and experimental research which are of a high standard and which contribute to the advancement of knowledge in the field of hypertension. The Journal publishes full papers, reviews or editorials (normally by invitation), and correspondence.
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