Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Xin Deng, Hui Liu, Wei Zhao, Rui Wu, Kuo Chen, Qing Li, Murat Onur Yazlık, Hüseyin Özkan, Jingyuan Ren, Jiacheng Zhang, Shiyi Liu, Ling Mei, Shangfeng Li, Jiasui Zhan, Binhong Hu
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Abstract

The pathogenesis of canine pyometra is multifactorial, involving hormonal imbalances, aberrant immune responses, and metabolic dysregulation includes lipid metabolism and oxidative stress. This study focuses on lipid metabolism and oxidative stress, revealing the key regulatory role of AMPK and PLIN2 in canine pyometra. Bitches with open cervix pyometra (n:8) and healthy bitches undergoing elective ovariohysterectomy (n:4) were enrolled to the study. In experiment one, the serum and tissue levels of Malondialdehyde (MDA) and Superoxide Dismutase (SOD) activity were assessed. Additionally, uterine histopathological analysis, AMPK and PLIN2 expressions were determined through immunohistochemistry. Furthermore, inflammation, oxidative stress, and lipid metabolism-related factors were evaluated using Western blot analysis. In experiment two, primary cell cultures were prepared from healthy uterine endometrial cells of the dogs in control group. Cultured canine endometrial epithelial cells were treated with lipopolysaccharide (LPS) along with oleic acid (OA) to induce an inflammatory response. Tissue and serum MDA and SOD levels were greater in dogs with pyometra. Accumulated lipid droplets were observed in the uterine tissue of bitches with pyometra. The phosphorylation of AMPK and the expression of PLIN2 significantly increased in the pyometra group. The expression of related lipid synthesis proteins such as ACC1, FASN, SREBP-1c, and PLIN2 was upregulated, while PPARα and PGC1α were downregulated in bitches with pyometra. In experiment two, activation of AMPK and PLIN2 not only restores the expression of PGC1α, but also effectively alleviates inflammation and oxidative stress. The role of lipid metabolism and oxidative stress in canine pyometra is elucidated, thus contributing to the pathogenesis of pyometra in dogs.

AMPK和PLIN2的表达在开放宫颈脓膜母狗脂质代谢和氧化应激中的调控作用。
犬脓脓的发病机制是多因素的,包括激素失衡、异常免疫反应和代谢失调,包括脂质代谢和氧化应激。本研究着眼于脂质代谢和氧化应激,揭示AMPK和PLIN2在犬脓脓症中的关键调控作用。有开放宫颈脓腔的母狗(8只)和接受择期卵巢子宫切除术的健康母狗(4只)被纳入研究。试验一测定血清和组织中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性。免疫组化检测AMPK、PLIN2的表达。此外,使用Western blot分析评估炎症、氧化应激和脂质代谢相关因素。实验二,以对照组犬的健康子宫内膜细胞制备原代细胞培养物。用脂多糖(LPS)和油酸(OA)处理体外培养的犬子宫内膜上皮细胞,诱导炎症反应。脓脓狗的组织和血清MDA和SOD水平较高。脓膜炎母狗子宫组织中可见脂滴积聚。脓膜组AMPK磷酸化和PLIN2表达显著升高。脓脓母狗相关脂质合成蛋白ACC1、FASN、SREBP-1c、PLIN2表达上调,PPARα、PGC1α表达下调。在实验二中,激活AMPK和PLIN2不仅可以恢复PGC1α的表达,还可以有效缓解炎症和氧化应激。脂质代谢和氧化应激在犬脓脓病中的作用被阐明,从而有助于犬脓脓病的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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