Comparative evaluation of three herbal extracts on growth performance, immune response, and resistance against Vibrio parahaemolyticus in Litopenaeus vannamei.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Amr Fadel, Amal Khafage, Mohamed Abdelsalam, Mohamed M Abdel-Rahim
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引用次数: 0

Abstract

This study evaluated the effects of dietary supplementation with herbal extracts from Artemisia herba-alba, Lonicera japonica, and Lilium candidum on growth performance, survival, feed utilization, antioxidant capacity, and immune response in Litopenaeus vannamei. The efficacy of these herbal-supplemented diets was assessed in enhancing resistance against Vibrio parahaemolyticus-induced Acute Hepatopancreatic Necrosis Disease (Vp AHPND). A total of 2,400 shrimp post-larvae (initial weight 0.74 ± 0.02 g) were randomly assigned to four triplicate groups. Shrimp were fed isonitrogenous and isocaloric diets: T1 (control, basal diet), T2 (basal diet + 250 mg/kg A. herba-alba), T3 (basal diet + 250 mg/kg L. japonica), and T4 (basal diet + 250 mg/kg L. candidum). Herbal-supplemented groups showed significantly improved (P ≤ 0.05) growth performance, feed utilization, and survival rates compared to the control, with T4 exhibiting the highest values. Significant enhancements of immune assays were observed in total hemocyte count, phagocytosis activity, total protein, glutathione peroxidase, and lysozyme activity in herbal-supplemented groups. Antioxidant indicators (catalase, superoxide dismutase, and phenoloxidase) were boosted while malondialdehyde levels decreased in herbal-treated shrimp. Following V. parahaemolyticus challenge, herbal diets effectively reduced cumulative mortality in L. vannamei. Histopathological examination revealed milder AHPND-associated alterations in A. herba-alba and L. candidum-treated groups, contrasting with atrophy, necrosis, and epithelial cell sloughing observed in the positive control. These findings demonstrate the immunostimulatory potential of A. herba-alba, L. japonica, and L. candidum as dietary supplements to enhance growth performance, immune function, and disease resistance in L. vannamei aquaculture, offering a promising strategy for sustainable shrimp farming.

三种草药提取物对凡纳滨对虾生长性能、免疫反应及对副溶血性弧菌抗性的比较评价。
本研究评价了饲粮中添加白蒿、金银花和白百合提取物对凡纳滨对虾生长性能、存活率、饲料利用率、抗氧化能力和免疫反应的影响。评估了这些草药补充饮食在增强对副溶血性弧菌诱导的急性肝胰腺坏死病(Vp AHPND)的抵抗力方面的功效。选取2400尾初始体重为0.74±0.02 g的稚虾,随机分为4个3重复组。饲喂等氮等热量饲料:T1(对照,基础饲粮)、T2(基础饲粮+ 250 mg/kg白花海参)、T3(基础饲粮+ 250 mg/kg粳稻)和T4(基础饲粮+ 250 mg/kg白花海参)。与对照组相比,草药添加组的生长性能、饲料利用率和成活率均显著提高(P≤0.05),其中T4含量最高。在总血细胞计数、吞噬活性、总蛋白、谷胱甘肽过氧化物酶和溶菌酶活性方面,添加草药组的免疫检测结果显著增强。抗氧化指标(过氧化氢酶、超氧化物歧化酶和酚氧化酶)在草药处理的虾中提高,而丙二醛水平降低。在副溶血性弧菌攻击后,草药饮食有效地降低了凡纳美弧菌的累积死亡率。组织病理学检查显示,与阳性对照中观察到的萎缩、坏死和上皮细胞脱落相比,白草单胞菌和念珠菌处理组ahpnd相关的改变较轻。这些研究结果表明,在凡纳滨对虾养殖中,作为饲料添加剂的白花对虾、粳稻对虾和铁皮对虾具有提高生长性能、免疫功能和抗病能力的免疫刺激潜力,为可持续对虾养殖提供了一种有前景的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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