Comparative evaluation of three herbal extracts on growth performance, immune response, and resistance against Vibrio parahaemolyticus in Litopenaeus vannamei.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Amr Fadel, Amal Khafage, Mohamed Abdelsalam, Mohamed M Abdel-Rahim
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引用次数: 0

Abstract

This study evaluated the effects of dietary supplementation with herbal extracts from Artemisia herba-alba, Lonicera japonica, and Lilium candidum on growth performance, survival, feed utilization, antioxidant capacity, and immune response in Litopenaeus vannamei. The efficacy of these herbal-supplemented diets was assessed in enhancing resistance against Vibrio parahaemolyticus-induced Acute Hepatopancreatic Necrosis Disease (Vp AHPND). A total of 2,400 shrimp post-larvae (initial weight 0.74 ± 0.02 g) were randomly assigned to four triplicate groups. Shrimp were fed isonitrogenous and isocaloric diets: T1 (control, basal diet), T2 (basal diet + 250 mg/kg A. herba-alba), T3 (basal diet + 250 mg/kg L. japonica), and T4 (basal diet + 250 mg/kg L. candidum). Herbal-supplemented groups showed significantly improved (P ≤ 0.05) growth performance, feed utilization, and survival rates compared to the control, with T4 exhibiting the highest values. Significant enhancements of immune assays were observed in total hemocyte count, phagocytosis activity, total protein, glutathione peroxidase, and lysozyme activity in herbal-supplemented groups. Antioxidant indicators (catalase, superoxide dismutase, and phenoloxidase) were boosted while malondialdehyde levels decreased in herbal-treated shrimp. Following V. parahaemolyticus challenge, herbal diets effectively reduced cumulative mortality in L. vannamei. Histopathological examination revealed milder AHPND-associated alterations in A. herba-alba and L. candidum-treated groups, contrasting with atrophy, necrosis, and epithelial cell sloughing observed in the positive control. These findings demonstrate the immunostimulatory potential of A. herba-alba, L. japonica, and L. candidum as dietary supplements to enhance growth performance, immune function, and disease resistance in L. vannamei aquaculture, offering a promising strategy for sustainable shrimp farming.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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