Identification and characterisation of LEAP2 from Chinese spiny frogs (Quasipaa spinosa) with antimicrobial and macrophage activation properties.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Ping Ying, Xin-Yi Qian, Zi-Xuan Wang, Jia-Le Wu, Jia-Yin Huang, Zi-Yi Ren, Jie Chen
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Abstract

Background: The liver-expressed antimicrobial peptide 2 (LEAP2) family is an important group of antimicrobial peptides (AMPs) involved in vertebrate defence against bacterial infections. However, research on LEAP2 in amphibians is still in its infancy.

Results: This study aimed to explore the role of LEAP2 in the Chinese spiny frog (Quasipaa spinosa). The cDNA of the LEAP2 gene (QsLEAP2) was cloned from a Chinese spiny frog. The QsLEAP2 protein comprises a signal peptide, a prodomain, and a mature peptide. Sequence analysis indicated that QsLEAP2 is a member of the amphibian LEAP2 cluster and closely related to the LEAP2 of the African clawed frog (Xenopus laevis). Expression of QsLEAP2 was detected in various tissues, with the liver exhibiting the highest expression. Following infection with Aeromonas hydrophila, QsLEAP2 expression was significantly upregulated in the spleen, lungs, kidneys, liver, and gut. The synthetic mature peptide QsLEAP2 exhibited selective antimicrobial activity against several bacterial strains in vitro. It disrupted bacterial membrane integrity and hydrolysed bacterial genomic DNA, exhibiting bactericidal effects on specific bacterial species. Furthermore, QsLEAP2 induced chemotaxis in RAW264.7 murine leukemic monocytes/macrophages, enhancing their phagocytic activity and respiratory bursts. Docking simulations revealed an interaction between QsLEAP2 and QsMOSPD2.

Conclusions: These findings provide new insights into the role of LEAP2 in the amphibian immune system.

中国棘蛙(Quasipaa spinosa)中具有抗菌和巨噬细胞活化特性的LEAP2的鉴定和表征。
背景:肝脏表达的抗菌肽2 (LEAP2)家族是一组重要的抗菌肽(amp),参与脊椎动物对细菌感染的防御。然而,对两栖动物中LEAP2的研究仍处于起步阶段。结果:本研究旨在探讨LEAP2在中国棘蛙(Quasipaa spinosa)中的作用。从中国刺蛙中克隆了LEAP2基因(QsLEAP2)的cDNA。QsLEAP2蛋白包括一个信号肽、一个前结构域和一个成熟肽。序列分析表明,QsLEAP2是两栖动物LEAP2群的成员,与非洲爪蛙(Xenopus laevis)的LEAP2亲缘关系密切。QsLEAP2在各组织中均有表达,其中肝脏表达量最高。感染嗜水气单胞菌后,QsLEAP2在脾、肺、肾、肝和肠道中的表达显著上调。合成的成熟肽QsLEAP2在体外对多种细菌具有选择性抗菌活性。它破坏细菌膜的完整性并水解细菌基因组DNA,对特定细菌种类表现出杀菌作用。此外,QsLEAP2诱导RAW264.7小鼠白血病单核/巨噬细胞趋化,增强其吞噬活性和呼吸爆发。对接模拟揭示了QsLEAP2和QsMOSPD2之间的相互作用。结论:这些发现对LEAP2在两栖动物免疫系统中的作用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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