Clinicopathological Features and HOX Transcript Antisense RNA In Situ Hybridization Detection of Myxopapillary Ependymoma.

Q4 Medicine
Zhang Yu-Han, Wang Zheng, L U Jun-Liang, Zhao Da-Chun, Huo Zhen
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引用次数: 0

Abstract

Objective To summarize the clinicopathological features,immunohistochemical characteristics,HOX transcript antisense RNA(HOTAIR)in situ hybridization status,treatment,and prognosis of myxopapillary ependymoma(MPE). Methods A total of 17 patients diagnosed with MPE based on pathological evidence in the Department of Pathology of Peking Union Medical College Hospital from November 2006 to July 2023 were selected,and the clinicopathological data of these patients were collected.Immunohistochemical staining for trimethylation at lysine 27 of histone H3 (H3K27me3),glial fibrillary acidic protein(GFAP),and epithelial membrane antigen(EMA)and alcian blue-periodic acid Schiff(AB-PAS)staining were performed in all the patients.Sixteen patients with spinal ependymomas were selected as the control group.Tissue microarrays were prepared from 17 MPE patients and the control group.HOTAIR ISH was performed and semi-quantitatively scored,and the scores of the two groups were compared by the Wilcoxon rank-sum test. Results The 17 MPE patients aged 14-64 years,with the mean age of(37.48±16.10)years and the male-to-female ratio of 0.7∶1.Their clinical manifestations mainly included lumbosacral and lower limb pains.Microscopically,tumor cells were arranged in a papillary pattern around fibrovascular axis,with abundant myxoid materials,and tumor cells were arranged in a loose meshwork in some patients.The immunohistochemical staining results showed that 17(100%),10(58.82%),and 8(47.06%)patients expressed GFAP,EMA,and D2-40,respectively,and 2(11.76%)patients lacked expression of H3K27me3.AB-PAS staining showed blue myxoid materials in all the 17(100%)patients.HOTAIR was expressed in both MPE and control groups,with higher semi-quantitative score in the MPE group than in the control group(P=0.004).Twelve patients were followed up,with a median follow-up period of 65.50 months,during which three patients showed recurrence.Conclusions MPE exhibits typical pathological features,and the combination with immunohistochemical staining for GFAP and EMA as well as AB-PAS staining facilitates diagnosis of this disease.A small number of patients loss the expression of H3K27me3.HOTAIR is highly expressed in MPE but lacks specificity,which limits its auxiliary diagnostic value.The overall prognosis of MPE is favorable,with a few patients experiencing recurrence.

摘要] 目的 总结肌乳头状上皮瘤(MPE)的临床病理特征、免疫组化特点、HOX转录物反义RNA(HOTAIR)原位杂交情况、治疗及预后。方法 选择 2006 年 11 月至 2023 年 7 月在北京协和医院病理科根据病理证据确诊的 17 例 MPE 患者,收集其临床病理资料。所有患者均进行了组蛋白 H3 赖氨酸 27 三甲基化(H3K27me3)、神经胶质纤维酸性蛋白(GFAP)、上皮膜抗原(EMA)的免疫组化染色和藻蓝-碘酸希夫(AB-PAS)染色。制备 17 例 MPE 患者和对照组的组织芯片,进行 HOTAIR ISH 半定量评分,两组评分比较采用 Wilcoxon 秩和检验。结果 17例MPE患者年龄在14-64岁之间,平均年龄为(37.48±16.10)岁,男女比例为0.7∶1,临床表现主要为腰骶部和下肢疼痛,显微镜下肿瘤细胞围绕纤维血管轴呈乳头状排列,内含丰富的肌样物质,部分患者肿瘤细胞呈疏松网状排列。免疫组化染色结果显示,17 例(100%)、10 例(58.82%)和 8 例(47.06%)患者分别表达 GFAP、EMA 和 D2-40,2 例(11.76%)患者缺乏 H3K27me3 表达。HOTAIR在MPE组和对照组均有表达,MPE组的半定量评分高于对照组(P=0.004)。结论 MPE 具有典型的病理特征,结合 GFAP 和 EMA 免疫组化染色以及 AB-PAS 染色有助于该病的诊断,少数患者失去 H3K27me3 的表达,HOTAIR 在 MPE 中高表达,但缺乏特异性,这限制了其辅助诊断价值。
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来源期刊
中国医学科学院学报
中国医学科学院学报 Medicine-Medicine (all)
CiteScore
0.60
自引率
0.00%
发文量
6813
期刊介绍: Acta Academiae Medicinae Sinicae was founded in February 1979. It is a comprehensive medical academic journal published in China and abroad, supervised by the Ministry of Health of the People's Republic of China and sponsored by the Chinese Academy of Medical Sciences and Peking Union Medical College. The journal mainly reports the latest research results, work progress and dynamics in the fields of basic medicine, clinical medicine, pharmacy, preventive medicine, biomedicine, medical teaching and research, aiming to promote the exchange of medical information and improve the academic level of medicine. At present, the journal has been included in 10 famous foreign retrieval systems and their databases [Medline (PubMed online version), Elsevier, EMBASE, CA, WPRIM, ExtraMED, IC, JST, UPD and EBSCO-ASP]; and has been included in important domestic retrieval systems and databases [China Science Citation Database (Documentation and Information Center of the Chinese Academy of Sciences), China Core Journals Overview (Peking University Library), China Science and Technology Paper Statistical Source Database (China Science and Technology Core Journals) (China Institute of Scientific and Technological Information), China Science and Technology Journal Paper and Citation Database (China Institute of Scientific and Technological Information)].
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