Endothelial cell alterations in capillaries of adipose tissue from patients affected by lipedema

IF 4.2 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Obesity Pub Date : 2025-03-12 DOI:10.1002/oby.24244

Sandro Michelini, Stefania Greco, Nicola Vaia, Valeria Puleo, Pamela Pellegrino, Angelica Di Vincenzo, Serena Michelini, Karen L. Herbst, Gaia Goteri, Tonia Luca, Sergio Castorina, Antonio Giordano, Pasquapina Ciarmela, Saverio Cinti

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引用次数: 0

Abstract

Objective

This study aimed to evaluate adipose tissue of lipedema patients.

Methods

Gluteo-femoral (affected area) and interscapular (nonaffected area) adipose tissue from 10 lean patients affected by lipedema stage 1 to 2 was studied and compared with tissue from 10 patients with obesity and 12 lean patients.

Results

The main features were alterations of capillaries with wall thickening (p ≤ 0.0001), endothelial and pericyte hyperplasia (p = 0.03 and p = 0.004), hypodense areas in basal membrane, and endothelial degeneration with exfoliation of degenerated cells into the capillary lumen. Adipocytes were larger (hypertrophic) in affected (P ≤ 0.0001) and nonaffected (p = 0.0003) areas compared with those with obesity and who were lean (both p ≤ 0.0001). Frequently the cytoplasm of adipocytes contained massive deposition of calcium crystals as revealed by Von Kossa staining (p = 0.023) and electron microscopy. CD68 immunoreactive macrophages were more abundant in affected areas (p = 0.005), and their number was similar to that found in fat from patients with obesity (p = 0.17). Despite adipocyte hypertrophy and inflammation, lack of the healthy marker perilipin-1 and the presence of crown-like structures were only rarely seen, while they were quite frequent in patients with obesity.

Conclusions

Our data support the idea that cell alterations happen in the early stages of adipocyte development (endothelium/pericyte) in the adipose organ of women affected by lipedema.

Abstract Image

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脂水肿患者脂肪组织毛细血管内皮细胞的改变。

目的：研究脂水肿患者的脂肪组织。方法：对10例1 ~ 2期脂肪水肿患者的臀股（患区）和肩胛间（非患区）脂肪组织进行研究，并与10例肥胖患者和12例瘦弱患者的组织进行比较。结果：主要表现为毛细血管改变伴壁增厚（p≤0.0001），内皮细胞和周细胞增生（p = 0.03和p = 0.004），基底膜低密度区，内皮变性伴退变细胞脱落进入毛细血管管腔。与肥胖者和瘦者（P≤0.0001）相比，肥胖区（P≤0.0001）和非肥胖区（P = 0.0003）的脂肪细胞较大（肥厚）。Von Kossa染色（p = 0.023）和电镜检查显示，脂肪细胞细胞质常含有大量钙晶体沉积。CD68免疫反应性巨噬细胞在患处更丰富（p = 0.005），其数量与肥胖患者脂肪中的CD68免疫反应性巨噬细胞数量相似（p = 0.17）。尽管存在脂肪细胞肥大和炎症，但健康标志物periilipin -1的缺乏和冠状结构的存在只是罕见的，而在肥胖患者中却相当常见。结论：我们的数据支持这样一种观点，即脂肪水肿女性脂肪器官的细胞改变发生在脂肪细胞发育的早期阶段（内皮/周细胞）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Obesity 医学-内分泌学与代谢

CiteScore

11.70

自引率

1.40%

发文量

261

审稿时长

2-4 weeks

期刊介绍： Obesity is the official journal of The Obesity Society and is the premier source of information for increasing knowledge, fostering translational research from basic to population science, and promoting better treatment for people with obesity. Obesity publishes important peer-reviewed research and cutting-edge reviews, commentaries, and public health and medical developments.