Yavuz Şahin, Elif Sibel Aslan, Süleyman Aktuna, Volkan Baltacı
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引用次数: 0
Abstract
Objective: The transition nuclear protein 1 (TNP1) gene is a member of the TNP family and is abundantly expressed during spermatogenesis. Protamine 1 (PRM1), another sperm nuclear protein, is abundant in many species. The present study aimed to evaluate transition nuclear protein 1 (TNP1) and protamine 1 (PRM1) gene expression in infertile male patients with low and high sperm DNA fragmentation (SDF).
Material and methods: Semen samples (n=100) were obtained from male participants undertaking treatment with intracytoplasmic sperm injection. The expression levels of TNP1 and PRM1 were measured using real-time quantitative polymerase chain reaction. The data were compared with statistical tests, (independent samples T- or Mann-Whitney U) as appropriate. A p<0.05 was considered significant.
Results: Patients with low-SDF exhibited a significantly lower sperm concentration compared to those with high-SDF (p=0.002). There was significant down regulation of TNP1 (p=0.036) and PRM1 (p=0.04) in patients exhibiting high-SDF levels compared to those with low-SDF levels. A significant moderate positive correlation was observed between the relative expression levels of TNP1 and PRM1 (r=0.459, p<0.001).
Conclusion: In the present study TNP1 and PRM1 were differentially expressed in male patients being treated for infertility and who had low or high-SDF.
期刊介绍：
Journal of the Turkish-German Gynecological Association is the official, open access publication of the Turkish-German Gynecological Education and Research Foundation and Turkish-German Gynecological Association and is published quarterly on March, June, September and December. It is an independent peer-reviewed international journal printed in English language. Manuscripts are reviewed in accordance with “double-blind peer review” process for both reviewers and authors. The target audience of Journal of the Turkish-German Gynecological Association includes gynecologists and primary care physicians interested in gynecology practice. It publishes original works on all aspects of obstertrics and gynecology. The aim of Journal of the Turkish-German Gynecological Association is to publish high quality original research articles. In addition to research articles, reviews, editorials, letters to the editor, diagnostic puzzle are also published. Suggestions for new books are also welcomed. Journal of the Turkish-German Gynecological Association does not charge any fee for article submission or processing.
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