Development of NIR photocleavable nanoparticles with BDNF for vestibular neuron regeneration.

IF 10.6 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Celine Dg Abueva, Sung Ryeong Yoon, Nathaniel T Carpena, Seung Cheol Ahn, So-Young Chang, Ji Eun Choi, Min Young Lee, Jae Yun Jung
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Abstract

Among nanoparticle platforms, light or photoresponsive nanoparticles have emerged as a promising drug delivery strategy with spatiotemporal control while minimizing off-target effects. The characteristic absorption spectrum of the photoresponsive moiety dictates the wavelength of light needed to activate bond cleavage. However, the low tissue penetration depth limit and short-wavelength ultraviolet (UV) cellular toxicity are considered disadvantageous. This study developed a vestibular ganglion neuron organoid as a model for vestibulopathy. UV and near-infrared (NIR) radiation targeted the inner ear and neural cells, followed by toxicity evaluation. A significantly smaller toxicity of NIR light was confirmed. The photocleavage release of brain-derived neurotrophic factor (BDNF) was used by applying NIR wavelength. The results indicate that polyethylene glycol octamethylene diamine derivative conjugated with leucomethylene blue with an ethanolamine linker nanoparticle can be effectively disassembled and release BDNF when using the 808 nm laser as a trigger. The findings of the cytotoxicity assay suggest that photocleavable nanoparticles (PCNs) and laser irradiation are safe and biocompatible for human-derived and neural progenitor types of cells. Phototriggered BDNF release by NIR laser supported the growth and differentiation of human neural progenitor cells in culture. In addition, the vestibulopathy organoid exhibited a significant regenerative effect. This study harnesses the full potential of NIR laser PCNs to treat vestibular neuropathies.

用于前庭神经元再生的BDNF纳米颗粒的制备。
在纳米颗粒平台中,光或光响应纳米颗粒已成为一种有前途的药物递送策略,具有时空控制,同时最大限度地减少脱靶效应。光响应部分的特征吸收光谱决定了激活键裂解所需的光的波长。然而,较低的组织穿透深度限制和短波紫外线(UV)细胞毒性被认为是不利的。本研究开发了一个前庭神经节神经元类器官作为前庭病变的模型。紫外和近红外(NIR)辐射靶向内耳和神经细胞,然后进行毒性评估。证实近红外光的毒性明显较小。利用近红外波长对脑源性神经营养因子(BDNF)进行光解释放。结果表明,在808 nm激光触发下,聚乙二醇八亚甲基二胺衍生物与白乙烯蓝结合,乙醇胺连接纳米粒子可以有效地分解并释放BDNF。细胞毒性实验结果表明,光切割纳米颗粒(PCNs)和激光照射对于人源性和神经祖细胞类型是安全的和生物相容性的。近红外激光光触发BDNF释放支持培养的人神经祖细胞的生长和分化。此外,前庭病变类器官表现出显著的再生作用。本研究充分利用了近红外激光pcn治疗前庭神经病变的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Nanobiotechnology
Journal of Nanobiotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
13.90
自引率
4.90%
发文量
493
审稿时长
16 weeks
期刊介绍: Journal of Nanobiotechnology is an open access peer-reviewed journal communicating scientific and technological advances in the fields of medicine and biology, with an emphasis in their interface with nanoscale sciences. The journal provides biomedical scientists and the international biotechnology business community with the latest developments in the growing field of Nanobiotechnology.
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