{"title":"Spectrofluorophotometric Analysis of Phytoconstituents, Biomarkers, Enzyme Kinetics and Trace Metals: A Comprehensive Review.","authors":"Amol Warokar, Bhagyashree Dahake, Purushottam Gangane, Swapnil Samrit","doi":"10.1007/s10895-025-04241-x","DOIUrl":null,"url":null,"abstract":"<p><p>Spectrofluorophotometry is a highly sensitive and selective analytical technique widely employed in pharmaceutical, biomedical, and environmental sciences. This review provides a comprehensive application for detecting and quantifying phytoconstituents, biomarkers, enzyme kinetics, and trace metals. This technique offers detection of analyte in the femtomolar (fM) range. Phytochemicals such as flavonoids, alkaloids, and polyphenols were analyzed with high precision and accuracy, and the reported analytical method can be adopted for quality control analysis. Spectrofluorophotometry has been utilized to estimate biomarkers, which plays a critical role in disease diagnostics and therapeutic monitoring. It also facilitates the monitoring of enzyme kinetics, offering insights into metabolic processes and drug development. Additionally, its ability to detect trace metals through chelation and fluorescence quenching mechanisms proves vital for environmental and toxicological analysis. Despite challenges such as fluorescence quenching and environmental susceptibility, advancements in miniaturization, automation, and hybrid analytical techniques continue to enhance their capabilities. This review underscores the transformative impact of spectrofluorophotometry and its expanding role in modern analytical sciences.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04241-x","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Spectrofluorophotometry is a highly sensitive and selective analytical technique widely employed in pharmaceutical, biomedical, and environmental sciences. This review provides a comprehensive application for detecting and quantifying phytoconstituents, biomarkers, enzyme kinetics, and trace metals. This technique offers detection of analyte in the femtomolar (fM) range. Phytochemicals such as flavonoids, alkaloids, and polyphenols were analyzed with high precision and accuracy, and the reported analytical method can be adopted for quality control analysis. Spectrofluorophotometry has been utilized to estimate biomarkers, which plays a critical role in disease diagnostics and therapeutic monitoring. It also facilitates the monitoring of enzyme kinetics, offering insights into metabolic processes and drug development. Additionally, its ability to detect trace metals through chelation and fluorescence quenching mechanisms proves vital for environmental and toxicological analysis. Despite challenges such as fluorescence quenching and environmental susceptibility, advancements in miniaturization, automation, and hybrid analytical techniques continue to enhance their capabilities. This review underscores the transformative impact of spectrofluorophotometry and its expanding role in modern analytical sciences.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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