Protective effects of Paeonia suffruticosa callus extract in skin through anti-inflammation and repair UVB-induced damage

IF 2.5 4区 医学 Q2 DERMATOLOGY
Yufan Li, Jiejun Han, Rongyue Gong, Yuankun Liu, Yu Zhou, Tiangui Gong, Bin Wang, Laidi Zhang, Shuodan Li, Jiayue Chen
{"title":"Protective effects of Paeonia suffruticosa callus extract in skin through anti-inflammation and repair UVB-induced damage","authors":"Yufan Li,&nbsp;Jiejun Han,&nbsp;Rongyue Gong,&nbsp;Yuankun Liu,&nbsp;Yu Zhou,&nbsp;Tiangui Gong,&nbsp;Bin Wang,&nbsp;Laidi Zhang,&nbsp;Shuodan Li,&nbsp;Jiayue Chen","doi":"10.1111/ics.13055","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>The study investigated effects of peony callus extracts (PCE) on the protective efficacy against Ultraviolet B (UVB)-induced photoageing, using in vitro and in vivo studies. The research focused on PCE's ability to protect against inflammatory factors, DNA damage and accumulation of senescent cells, along with the evaluation of the extract's potential anti-photoageing benefits to skin.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Human keratinocyte cell line (HaCaT cells), mast cells and fibroblasts were used to evaluate the role of PCE in anti-photoageing. The expression of genes of <i>interleukin-1α (IL-1α)</i>, <i>IL-6</i> and transient receptor potential vanilloid 1 (<i>TRPV1</i>) were tested in HaCaT cells. The histamine contents in mast cells were tested to evaluate the effect of PCE on soothing skin. Additionally, the repairment of PCE on DNA damage stimulated by UVB using comet assay was evaluated. In fibroblasts, the gene expression of <i>matrix metalloproteinases</i> (<i>MMPs</i>) and the activity of β-galactosidase were tested. In vivo test, 13 healthy volunteers were enrolled to apply a formula with 1% PCE to assess the variation in inner skin collagen contents.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The callus from an ancient and rare variety of tree peony (<i>Paeoniaceae</i> family) was successfully induced, and its ingredients were extracted. The PCE could significantly downregulate inflammation factors such as <i>IL-1α</i>, <i>IL-6</i> and <i>TRPV1</i> in HaCaT cells, and <i>MMPs</i> in fibroblasts which could cause the collagen degradation induced by UVB. Meanwhile, UVB-induced DNA damage was alleviated by PCE. The analysis of histamine content in mast cells revealed that PCE effectively alleviated skin sensitivity. Furthermore, the clinical trials validated a significant increase in total collagen content in vivo, following 28 days of continuous application of a cosmetic formulation containing 1% PCE measured by Raman confocal spectroscopy technology.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The PCE could downregulate the gene expression of inflammatory factors, indicating the ability of DNA repair. The number of senescent cells was also decreased after UVB stimulation. Furthermore, the results of in vivo study showed that PCE was an ideal cosmetic ingredient for promoting collagen levels.</p>\n </section>\n </div>","PeriodicalId":13936,"journal":{"name":"International Journal of Cosmetic Science","volume":"47 4","pages":"652-664"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ics.13055","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Cosmetic Science","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ics.13055","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

The study investigated effects of peony callus extracts (PCE) on the protective efficacy against Ultraviolet B (UVB)-induced photoageing, using in vitro and in vivo studies. The research focused on PCE's ability to protect against inflammatory factors, DNA damage and accumulation of senescent cells, along with the evaluation of the extract's potential anti-photoageing benefits to skin.

Methods

Human keratinocyte cell line (HaCaT cells), mast cells and fibroblasts were used to evaluate the role of PCE in anti-photoageing. The expression of genes of interleukin-1α (IL-1α), IL-6 and transient receptor potential vanilloid 1 (TRPV1) were tested in HaCaT cells. The histamine contents in mast cells were tested to evaluate the effect of PCE on soothing skin. Additionally, the repairment of PCE on DNA damage stimulated by UVB using comet assay was evaluated. In fibroblasts, the gene expression of matrix metalloproteinases (MMPs) and the activity of β-galactosidase were tested. In vivo test, 13 healthy volunteers were enrolled to apply a formula with 1% PCE to assess the variation in inner skin collagen contents.

Results

The callus from an ancient and rare variety of tree peony (Paeoniaceae family) was successfully induced, and its ingredients were extracted. The PCE could significantly downregulate inflammation factors such as IL-1α, IL-6 and TRPV1 in HaCaT cells, and MMPs in fibroblasts which could cause the collagen degradation induced by UVB. Meanwhile, UVB-induced DNA damage was alleviated by PCE. The analysis of histamine content in mast cells revealed that PCE effectively alleviated skin sensitivity. Furthermore, the clinical trials validated a significant increase in total collagen content in vivo, following 28 days of continuous application of a cosmetic formulation containing 1% PCE measured by Raman confocal spectroscopy technology.

Conclusion

The PCE could downregulate the gene expression of inflammatory factors, indicating the ability of DNA repair. The number of senescent cells was also decreased after UVB stimulation. Furthermore, the results of in vivo study showed that PCE was an ideal cosmetic ingredient for promoting collagen levels.

Abstract Image

白芍愈伤组织提取物对皮肤抗炎修复uvb损伤的保护作用。
目的:通过体外和体内实验研究牡丹愈伤组织提取物(PCE)对紫外线B (UVB)诱导的光老化的保护作用。这项研究的重点是PCE对炎症因子、DNA损伤和衰老细胞积累的保护能力,以及对提取物对皮肤的潜在抗光老化作用的评估。方法:采用人角化细胞(HaCaT)、肥大细胞和成纤维细胞观察PCE的抗光老化作用。检测HaCaT细胞中白细胞介素-1α (IL-1α)、IL-6和瞬时受体电位香草样蛋白1 (TRPV1)基因的表达。检测肥大细胞组胺含量,评价PCE对皮肤的舒缓作用。此外,采用彗星法评价PCE对UVB刺激下DNA损伤的修复作用。在成纤维细胞中检测基质金属蛋白酶(MMPs)的基因表达和β-半乳糖苷酶的活性。在体内试验中,13名健康志愿者使用含有1% PCE的配方来评估内皮胶原蛋白含量的变化。结果:成功诱导了一种古老而稀有的芍药品种愈伤组织,并提取了其成分。PCE可显著下调HaCaT细胞中的炎症因子IL-1α、IL-6、TRPV1以及成纤维细胞中的MMPs,这些因子可引起UVB诱导的胶原降解。同时,PCE可减轻uvb诱导的DNA损伤,肥大细胞组胺含量分析显示PCE可有效减轻皮肤敏感性。此外,临床试验证实,在连续使用含有1% PCE的化妆品配方28天后,体内总胶原含量显著增加,该化妆品配方通过拉曼共聚焦光谱技术测量。结论:PCE可下调炎症因子基因表达,提示其具有DNA修复能力。UVB刺激后衰老细胞数量减少。此外,体内研究结果表明,PCE是一种理想的促进胶原蛋白水平的化妆品成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.60
自引率
4.30%
发文量
73
期刊介绍: The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信