Enhancing drought tolerance in Pisum sativum and Vicia faba through interspecific interactions with a mixed inoculum of Rhizobium laguerreae and non-host beneficial rhizobacteria.
Asma Hami, Imane El Attar, Najoua Mghazli, Salah Ennajeh, El Houcine Ait-Ouakrim, Meryeme Bennis, Said Oulghazi, Bouabid Badaoui, Jamal Aurag, Laila Sbabou, Kaoutar Taha
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引用次数: 0
Abstract
Introduction: Harnessing plant growth-promoting rhizobia presents a sustainable and cost-effective method to enhance crop performance, particularly under drought stress. This study evaluates the variability of plant growth-promoting (PGP) traits among three strains of Rhizobium laguerreae LMR575, LMR571, and LMR655, and two native PGP strains Bacillus LMR698 and Enterobacter aerogenes LMR696. The primary objective was to assess the host range specificity of these strains and their effectiveness in improving drought tolerance in three legume species: Pisum sativum, Vicia faba, and Phaseolus vulgaris.
Methods: In-vitro experiments were conducted to assess the PGP traits of the selected strains, including phosphate solubilization, indole-3-acetic acid (IAA) production, and siderophore production. Greenhouse trials were also performed using a mixed inoculum of performing strains to evaluate their effects on plant physiological and biochemical traits under drought conditions.
Results: Significant variability in PGP traits was observed among the strains. R. laguerreae LMR655 exhibited the highest phosphate solubilization (113.85 mg mL-1 PO42-), while R. laguerreae LMR571 produced the highest IAA concentration (25.37 mg mL-1). E. aerogenes LMR696 demonstrated 82% siderophore production. Symbiotic interactions varied, with R. laguerreae LMR571 and LMR655 forming associations with P. sativum and V. faba, but none establishing compatibility with P. vulgaris. Greenhouse experiments showed that a mixed inoculum of R. laguerreae LMR571, LMR655, and E. aerogenes LMR696 significantly improved proline, total soluble sugars, proteins, and chlorophyll content under drought stress, with V. faba showing the strongest response.
Discussion: These findings highlight the importance of strain selection based on host specificity and PGP potential. The enhanced drought tolerance observed suggests that tailored microbial inoculants can improve legume resilience in water-limited environments. This study provides valuable insights for optimizing bioinoculant formulations to enhance crop performance under drought stress.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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