Research Progress on Neural Cell Culture Systems.

IF 4.8 2区 医学 Q1 NEUROSCIENCES
Ting Li, Xiaosong Qin, Qiang Ao
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引用次数: 0

Abstract

The nervous system, including the central nervous system and peripheral nervous system, has the most intricate structure and function among all systems in the human body. In studies of physiological and pathological functions, cell culture systems serve as an indispensable tool to simulate the nervous system in vivo. Two-dimensional (2D), three-dimensional (3D), and four-dimensional (4D) neural cell culture systems are used to assess the functional interconnectivity of neuronal tissues and have markedly advanced in recent years. Although 2D culture systems have predominated, they cannot accurately recapitulate the dynamic complexity of the in vivo environment, cell-cell communication, and nervous system structures. Consequently, studies have shifted to using 3D or 4D cell culture systems to achieve more realistic biochemical and biomechanical microenvironments. Nevertheless, many limitations persist in 3D or 4D culture systems, including difficulties in deciphering dynamic and reciprocal remodeling processes, as well as the spatiotemporal distributions of oxygen, nutrients, and metabolic waste. Here, we review 2D, 3D, and 4D culture systems, discuss the advantages and limitations of these techniques in modeling physiologically and pathologically relevant processes, and suggest directions for future research.

神经细胞培养系统的研究进展。
神经系统包括中枢神经系统和周围神经系统,在人体所有系统中具有最复杂的结构和功能。在生理和病理功能的研究中,细胞培养系统是模拟体内神经系统不可缺少的工具。近年来,二维(2D)、三维(3D)和四维(4D)神经细胞培养系统被用于评估神经组织的功能互联性,并取得了显著进展。虽然2D培养系统占主导地位,但它们不能准确地概括体内环境、细胞间通信和神经系统结构的动态复杂性。因此,研究已经转向使用3D或4D细胞培养系统来实现更真实的生化和生物力学微环境。然而,3D或4D培养系统仍然存在许多局限性,包括难以破译动态和互惠重塑过程,以及氧气、营养物质和代谢废物的时空分布。在此,我们回顾了2D、3D和4D培养系统,讨论了这些技术在模拟生理和病理相关过程中的优势和局限性,并提出了未来的研究方向。
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来源期刊
Current Neuropharmacology
Current Neuropharmacology 医学-神经科学
CiteScore
8.70
自引率
1.90%
发文量
369
审稿时长
>12 weeks
期刊介绍: Current Neuropharmacology aims to provide current, comprehensive/mini reviews and guest edited issues of all areas of neuropharmacology and related matters of neuroscience. The reviews cover the fields of molecular, cellular, and systems/behavioural aspects of neuropharmacology and neuroscience. The journal serves as a comprehensive, multidisciplinary expert forum for neuropharmacologists and neuroscientists.
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