Preparation and characterization of a certified reference material of toxic elements in cannabis leaves.

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Adriana Rodriguez, Cristhian Paredes, Elianna Castillo
{"title":"Preparation and characterization of a certified reference material of toxic elements in cannabis leaves.","authors":"Adriana Rodriguez, Cristhian Paredes, Elianna Castillo","doi":"10.1007/s00216-025-05809-z","DOIUrl":null,"url":null,"abstract":"<p><p>The quantification of harmful elements in cannabis is a relevant analytical task that requires metrological tools to ensure the reliability of the measurement results. This work reports the preparation and characterization of a certified reference material (CRM) for toxic elements-arsenic (As), cadmium (Cd), and lead (Pb)-in pulverized, lyophilized cannabis leaf tissue. To prepare the CRM INM-040-1, a portion of dried ground cannabis vegetal material was spiked with the elements at mass fraction values close to the levels of interest in relevant regulations for this kind of matrix: 0.34 mg/kg for arsenic, 0.34 mg/kg for cadmium, and 0.66 mg/kg for lead. The elements were quantified by inductively coupled plasma mass spectrometry (ICP-MS) in combination with graphite furnace atomic absorption spectroscopy (GF-AAS, for cadmium and lead) or hydride generation atomic absorption spectroscopy (HG-AAS, for arsenic). The analytical calibration was done by gravimetric standard addition for ICP-MS and GF-AAS, while bracketing calibration was used for HG-AAS. Furthermore, internal standard correction was used during ICP-MS measurements. The analytical methods were validated to demonstrate their fitness for purpose. The preparation variables of the CRM (particle size, drying treatment, and spiking conditions) were studied to improve the homogeneity of the CRM. The mass fraction of the toxic elements was certified with relative standard uncertainties ranging from 4.2 to 6.9%. The uncertainty contributions considered were the elements' mass fraction measurements, the between-methods bias, the (in)homogeneity of the production batch, and the (in)stability under transport and storage conditions. This new CRM constitutes a useful tool for the laboratories assessing the harmlessness of cannabis materials, promoting humans' safety and regulatory compliance within the medicinal cannabis industry.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00216-025-05809-z","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

The quantification of harmful elements in cannabis is a relevant analytical task that requires metrological tools to ensure the reliability of the measurement results. This work reports the preparation and characterization of a certified reference material (CRM) for toxic elements-arsenic (As), cadmium (Cd), and lead (Pb)-in pulverized, lyophilized cannabis leaf tissue. To prepare the CRM INM-040-1, a portion of dried ground cannabis vegetal material was spiked with the elements at mass fraction values close to the levels of interest in relevant regulations for this kind of matrix: 0.34 mg/kg for arsenic, 0.34 mg/kg for cadmium, and 0.66 mg/kg for lead. The elements were quantified by inductively coupled plasma mass spectrometry (ICP-MS) in combination with graphite furnace atomic absorption spectroscopy (GF-AAS, for cadmium and lead) or hydride generation atomic absorption spectroscopy (HG-AAS, for arsenic). The analytical calibration was done by gravimetric standard addition for ICP-MS and GF-AAS, while bracketing calibration was used for HG-AAS. Furthermore, internal standard correction was used during ICP-MS measurements. The analytical methods were validated to demonstrate their fitness for purpose. The preparation variables of the CRM (particle size, drying treatment, and spiking conditions) were studied to improve the homogeneity of the CRM. The mass fraction of the toxic elements was certified with relative standard uncertainties ranging from 4.2 to 6.9%. The uncertainty contributions considered were the elements' mass fraction measurements, the between-methods bias, the (in)homogeneity of the production batch, and the (in)stability under transport and storage conditions. This new CRM constitutes a useful tool for the laboratories assessing the harmlessness of cannabis materials, promoting humans' safety and regulatory compliance within the medicinal cannabis industry.

求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信