Influence of arbuscular mycorrhizal fungi on morpho-biochemical characteristics, nutrient uptake, and transcriptomic profile of Solanum melongena L. plant.

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-10 DOI:10.1007/s13205-025-04247-z

Subhesh Saurabh Jha, L S Songachan

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引用次数: 0

Abstract

The present study was carried out to observe the interaction between Solanum melongena L. with AMF treatments at morphological, biochemical, and molecular levels. The S. melongena plant was inoculated with Funneliformis mosseae, Glomus macrocarpum, Glomus monosporum, Acaulospora koskei, Acaulospora mellea and Rhizophagus irregularis, and also two consortia of F. mosseae + G. monosporum and F. mosseae + G. macrocarpum. Morphological and biochemical parameters such as shoot height, root length, shoot fresh weight, shoot dry weight, fruit fresh weight, fruit dry weight, root fresh weigh, root dry weight, chlorophyll a and b, total soluble sugars, total soluble proteins were examined in this study. Uptake of some essential micronutrients was also analyzed in this study. In transcriptomics experiment, a total of 365 transcripts were commonly upregulated (≥ 1.5x) folds in S. melongena in response to both the AMF isolates, while 44 transcripts were upregulated only in response to G. Macrocarpum and 28 transcripts were upregulated only in response to F. mosseae. KEGG pathway analysis of S. melongena treated with G. macrocarpum revealed carbon metabolism, cofactor biosynthesis and endocytosis as the dominant metabolic pathway, while analysis of F. mosseae treatment revealed glycerophospholipid and endocytosis metabolism as dominant metabolic pathways.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04247-z.

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丛枝菌根真菌对茄类植物形态生化特征、营养吸收和转录组学的影响

本研究在形态、生化和分子水平上观察了AMF处理对茄的相互作用。以黑麦球囊菌（Glomus macrocarpum）、单孢球囊菌（Glomus monosporum）、高斯球囊菌（Acaulospora koskei）、mellea球囊菌（Acaulospora mellea）和不规则根噬菌（Rhizophagus irregularis）以及两个F. mosseae + G菌群分别接种黑麦球囊菌。单孢菌和F. mosseae + G。macrocarpum。对茎高、根长、茎鲜重、茎干重、果实鲜重、果实干重、根鲜重、根干重、叶绿素a和b、总可溶性糖、总可溶性蛋白等形态生化参数进行了检测。本研究还分析了一些必需微量营养素的摄取情况。在转录组学实验中，两种AMF菌株对S. melongena共有365个转录本普遍上调（≥1.5倍）倍，而对G. Macrocarpum只上调44个转录本，对F. mosseae只上调28个转录本。KEGG途径分析显示，大角曲霉处理的黑葡萄球菌的主要代谢途径为碳代谢、辅因子生物合成和内吞作用，而F. mosseae处理的主要代谢途径为甘油磷脂代谢和内吞作用。补充信息：在线版本包含补充资料，提供地址为10.1007/s13205-025-04247-z。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.