Proteomic Analyses of Clots Identify Stroke Etiologies in Patients Undergoing Endovascular Therapy

IF 4.8 1区医学 Q1 NEUROSCIENCES

CNS Neuroscience & Therapeutics Pub Date : 2025-03-13 DOI:10.1111/cns.70340

Tae Jung Kim, Jin Woo Jung, Young-Ju Kim, Byung-Woo Yoon, Dohyun Han, Sang-Bae Ko

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Abstract

Aims

This study aimed to investigate the correlation between clot composition and stroke mechanisms in patients undergoing endovascular therapy (EVT), using proteomic analysis.

Methods

This study included 35 patients with ischemic stroke (cardioembolism [CE], n = 17; large artery atherosclerosis [LAA], n = 6; cancer-related [CR], n = 4; and undetermined (UD) cause, n = 8) who underwent EVT. Retrieved clots were proteomically analyzed to identify differentially expressed proteins associated with the three stroke mechanisms and to develop the machine learning model.

Results

In the discover stage, 3838 proteins were identified using clot samples from 27 patients with CE, LAA, and CR mechanisms. Through functional enrichment and network analysis, 149 proteins were identified as potential candidates for verification studies. After verification experiments, 34 proteins were selected as the final candidates to predict stroke mechanisms. Furthermore, the machine learning-based model identified three proteins associated with each mechanism (Pleckstrin in CE; CD59 glycoprotein in LAA; and Immunoglobulin Heavy Constant Gamma 1 in CR) in the UD group.

Conclusions

This study identified specific protein markers of clots that could differentiate stroke mechanisms in patients undergoing EVT. Therefore, our results could offer valuable insights into elucidating the mechanisms of ischemic stroke, which could provide information on more effective secondary prevention strategies.

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来源期刊

CNS Neuroscience & Therapeutics 医学-神经科学

CiteScore

7.30

自引率

12.70%

发文量

240

审稿时长

2 months

期刊介绍： CNS Neuroscience & Therapeutics provides a medium for rapid publication of original clinical, experimental, and translational research papers, timely reviews and reports of novel findings of therapeutic relevance to the central nervous system, as well as papers related to clinical pharmacology, drug development and novel methodologies for drug evaluation. The journal focuses on neurological and psychiatric diseases such as stroke, Parkinson’s disease, Alzheimer’s disease, depression, schizophrenia, epilepsy, and drug abuse.