{"title":"Adenium obesum as a New Natural Host of Pelargonium Flower Break Virus","authors":"Vinicius Henrique Bello, Tatsuya Nagata, Jorge Alberto Marques Rezende, Elliot Watanabe Kitajima","doi":"10.1111/jph.70052","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>In 2023, <i>Adenium obesum</i> plants displaying virus-like symptoms were observed in two locations in Brazil. High-throughput sequencing (HTS) analysis of RNA-Seq of one sample yielded a contig sequence of 3923 nucleotides (nt), showing 97.77% nt identity with the genome of pelargonium flower break virus (PFBV, genus <i>Alphacarmovirus</i>). Total RNA was also extracted from another sample, and the presence of PFBV was confirmed by the real-time reverse transcription-polymerase chain reaction (RT-qPCR) in these two samples. Attempts of mechanical transmission were done by inoculating sap from infected <i>A. obesum</i> leaves to healthy seedlings of <i>A. obesum</i> and <i>Pelargonium zonale</i>, but were not successful. However, PFBV was transmitted by grafting from and to an <i>A. obesum</i> plant. To the best of our knowledge, this is the first report of PFBV infecting <i>A. obesum</i> plants.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"173 2","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.70052","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
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Abstract
In 2023, Adenium obesum plants displaying virus-like symptoms were observed in two locations in Brazil. High-throughput sequencing (HTS) analysis of RNA-Seq of one sample yielded a contig sequence of 3923 nucleotides (nt), showing 97.77% nt identity with the genome of pelargonium flower break virus (PFBV, genus Alphacarmovirus). Total RNA was also extracted from another sample, and the presence of PFBV was confirmed by the real-time reverse transcription-polymerase chain reaction (RT-qPCR) in these two samples. Attempts of mechanical transmission were done by inoculating sap from infected A. obesum leaves to healthy seedlings of A. obesum and Pelargonium zonale, but were not successful. However, PFBV was transmitted by grafting from and to an A. obesum plant. To the best of our knowledge, this is the first report of PFBV infecting A. obesum plants.
期刊介绍：
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.
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