Microwaves Activate Immune Response and Promote Lymphocytes Proliferation of Wistar Rats

IF 2.2 4区医学 Q4 IMMUNOLOGY

Apmis Pub Date : 2025-03-14 DOI:10.1111/apm.70017

Ma Lizhen, Cao Shuhua, Zou Yong, Zhi Weijia, Zhao Xuelong, Zhang Mingzhao, Yan Zhifeng, Hu Xiangjun, Wang Lifeng

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引用次数: 0

Abstract

The potential effects of microwave radiation have gained increasing attention due to its widespread application in daily life. This study aimed to investigate its effects on rat immune function. Male Wistar rats were subjected to 2.856 GHz microwave radiation for 20 min. Cytokine levels in serum were measured by ELISA at 0 and 7 days' post-exposure. HE staining was used to observe the structure of the spleen. Morphology and proliferation of spleen mixed lymphocytes were detected after 24 h of culture, and proliferation was evaluated by the CCK-8 assay. Expression of HSP70 and calreticulin (CRT) was assessed by WB. Rats showed an increased secretion of inflammatory factors in serum. Spleen tissue revealed no significant damage. Compared to the control group, irradiated groups (R-0d and R-7d) showed a significant increase and thickening of the white pulp. The boundary between the red and white medulla in the R-7d group appeared blurred. CCK-8 assay showed that splenic mixed lymphocytes in the irradiated group showed a significant proliferation. There was no significant difference in HSP70 and CRT expression between the exposed and control groups. Microwave exposure might activate the immune system of rats, potentially triggering a T cell-mediated inflammatory response.

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微波激活免疫反应和促进Wistar大鼠淋巴细胞增殖

由于微波辐射在日常生活中的广泛应用，其潜在影响越来越受到人们的关注。本研究旨在探讨其对大鼠免疫功能的影响。雄性Wistar大鼠接受2.856 GHz微波辐射20分钟。暴露后0和7 d，采用ELISA法检测血清细胞因子水平。HE染色观察脾脏组织结构。培养24 h后检测脾脏混合淋巴细胞的形态和增殖情况，并采用CCK-8法评价其增殖情况。WB法检测HSP70和钙网蛋白（calreticulin， CRT）的表达。大鼠血清中炎性因子分泌增加。脾脏组织未见明显损伤。与对照组相比，辐照组（R-0d和R-7d）的白牙髓明显增加和增厚。R-7d组红髓质与白髓质界限模糊。CCK-8检测显示，照射组脾混合淋巴细胞明显增殖。暴露组与对照组HSP70和CRT的表达差异无统计学意义。微波暴露可能会激活大鼠的免疫系统，潜在地引发T细胞介导的炎症反应。

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来源期刊

Apmis 医学-病理学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： APMIS, formerly Acta Pathologica, Microbiologica et Immunologica Scandinavica, has been published since 1924 by the Scandinavian Societies for Medical Microbiology and Pathology as a non-profit-making scientific journal.