{"title":"Surface engineering of zirconia with abaloparatide and nano-hydroxyapatite for synergistic osteogenic activity","authors":"Hong Jae Lee, Yong-Dae Kwon, Sang Cheon Lee","doi":"10.1007/s13233-024-00336-9","DOIUrl":null,"url":null,"abstract":"<div><p>We report on the surface engineering of zirconia (ZrO<sub>2</sub>) by incorporation of abaloparatide (ABL) on the surface of nano-hydroxyapatite (nHAp)-immobilized zirconia. First, nHAp-immobilized zirconia (nHAp–PDA–ZrO<sub>2</sub>) was fabricated by adding nHAp in a polydopamine (PDA)-coating process on zirconia surface. Surface chemistry, morphology, and wettability of nHAp–PDA–ZrO<sub>2</sub> were examined using X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), and water contact angle measurement. As a second step, ABL incorporation was performed by the reaction of ABL with PDA layer coated on nHAp–PDA–ZrO<sub>2</sub>. A fluorescence microscope visualized that ABL was efficiently bound on the surface of nHAp–PDA–ZrO<sub>2</sub>to form ABL/nHAp–PDA–ZrO<sub>2</sub>. The synergistic osteogenic effect of ABL and nHAP on alkaline phosphatase (ALP) activity, calcium-mineral deposition, and alizarin red staining were assessed. The combination of ABL and nHAp for surface-engineering approaches may provide an effective approach to develop highly promising bone-regenerative osteogenic implant surfaces.</p><h3>Graphical abstract</h3><p>The surface of zirconia is engineered by incorporation of bone-forming abaloparatide (ABL) and osteoconductive nano-hydroxyapatite (nHAp). This ABL/nHAp-functionalized zirconia surface exhibits synergistic improved osteogenic activity.</p>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":688,"journal":{"name":"Macromolecular Research","volume":"33 3","pages":"277 - 287"},"PeriodicalIF":2.8000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Macromolecular Research","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s13233-024-00336-9","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
We report on the surface engineering of zirconia (ZrO2) by incorporation of abaloparatide (ABL) on the surface of nano-hydroxyapatite (nHAp)-immobilized zirconia. First, nHAp-immobilized zirconia (nHAp–PDA–ZrO2) was fabricated by adding nHAp in a polydopamine (PDA)-coating process on zirconia surface. Surface chemistry, morphology, and wettability of nHAp–PDA–ZrO2 were examined using X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), and water contact angle measurement. As a second step, ABL incorporation was performed by the reaction of ABL with PDA layer coated on nHAp–PDA–ZrO2. A fluorescence microscope visualized that ABL was efficiently bound on the surface of nHAp–PDA–ZrO2to form ABL/nHAp–PDA–ZrO2. The synergistic osteogenic effect of ABL and nHAP on alkaline phosphatase (ALP) activity, calcium-mineral deposition, and alizarin red staining were assessed. The combination of ABL and nHAp for surface-engineering approaches may provide an effective approach to develop highly promising bone-regenerative osteogenic implant surfaces.
Graphical abstract
The surface of zirconia is engineered by incorporation of bone-forming abaloparatide (ABL) and osteoconductive nano-hydroxyapatite (nHAp). This ABL/nHAp-functionalized zirconia surface exhibits synergistic improved osteogenic activity.
期刊介绍:
Original research on all aspects of polymer science, engineering and technology, including nanotechnology
Presents original research articles on all aspects of polymer science, engineering and technology
Coverage extends to such topics as nanotechnology, biotechnology and information technology
The English-language journal of the Polymer Society of Korea
Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.